Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju, 561-756, South Korea.
Appl Microbiol Biotechnol. 2010 Sep;88(1):283-9. doi: 10.1007/s00253-010-2755-4. Epub 2010 Jul 15.
Starvation induces significant alterations in lysosomal enzymes, and reduced concentrations of glucose increases the activity of several lysosomal enzymes. Therefore, to evaluate the lysosomal antimicrobial activity under starvation conditions, we added 0, 5, 10, 20, or 40 g/l of glucose (0%, 0.5%, 1%, 2%, or 4% glucose) supplemented YP medium to cultured Saccharomyces cerevisiae, and lysosomal fractions were isolated from S. cerevisiae grown under the various culture conditions. The lysosomes isolated from each condition exhibited increased antimicrobial activity against Escherichia coli as determined by a decrease in glucose concentration. In addition, a starvation-dependent increase in lysosomal activity coincided with increased lysosome intensity at the cytosol and distinct protein expression from lysosomes in S. cerevisiae. It also was determined found that the lysosomes have antimicrobial activity against seven different microorganisms, including E. coli, and starvation-induced lysosomes showed enhanced antimicrobial activity compared to those from normal lysosomes. These results suggest the possibility that lysosomal alterations during starvation may induce conditions that activate lysosomes for future development of efficient antimicrobial agents.
饥饿会引起溶酶体酶的显著变化，而葡萄糖浓度的降低会增加几种溶酶体酶的活性。因此，为了评估饥饿条件下溶酶体的抗菌活性，我们向培养的酿酒酵母中添加了 0、5、10、20 或 40 g/l 的葡萄糖（0%、0.5%、1%、2%或 4%葡萄糖）补充 YP 培养基，并从在各种培养条件下生长的酿酒酵母中分离出溶酶体部分。通过降低葡萄糖浓度，从每种条件分离出的溶酶体对大肠杆菌的抗菌活性增加。此外，溶酶体活性的饥饿依赖性增加与细胞质中溶酶体强度的增加以及酿酒酵母中溶酶体的独特蛋白表达一致。还发现溶酶体对包括大肠杆菌在内的七种不同微生物具有抗菌活性，饥饿诱导的溶酶体显示出比正常溶酶体更强的抗菌活性。这些结果表明，饥饿期间溶酶体的改变可能会引发激活溶酶体的条件，为未来开发有效的抗菌剂提供了可能性。
