Department of Medicine, Division of Geriatric Medicine and Gerontology, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA.
J Alzheimers Dis. 2010;21(3):769-73. doi: 10.3233/JAD-2010-100456.
Plasma amyloid-β (Aβ) level could be useful as a non-invasive biomarker in Alzheimer's disease research. We compared a multiplex electrochemiluminescence detection method with a well established ELISA method for plasma Aβ quantification. Compared to the ELISA method, the electrochemiluminescence detection method demonstrates a statistically significant, but modest correlation. The reasons for this may include the differences in the affinities of antibodies, and purity and source of Aβ peptides used as standards. However, the advantages of electrochemiluminescence detection technology include short processing time and small sample volume. This comparison demonstrates the need for a further study in optimizing this system.
血浆淀粉样蛋白-β(Aβ)水平可用作阿尔茨海默病研究中的一种非侵入性生物标志物。我们比较了一种多重电化学发光检测方法和一种成熟的 ELISA 方法,用于定量检测血浆 Aβ。与 ELISA 方法相比,电化学发光检测方法显示出具有统计学意义的,但适度的相关性。其原因可能包括抗体的亲和力、Aβ肽的纯度和来源的差异,这些都被用作标准。然而,电化学发光检测技术的优势包括处理时间短和样本量小。这项比较表明需要进一步研究来优化该系统。