[用甲基噻唑基四氮唑比色法对变形链球菌、血链球菌、伴放线放线杆菌计数的研究]

[Study on the counting of Streptococcus mutans, Streptococcus sanguis, Haemophilus actinomycetemcomitans by methyl thiazolyl tetrazolium colorimetric method].

作者信息

Wang Zhong-Chao, Fan Li-Yuan, Jiang Jun-Qiang, Cai Wei, Ding Yi

机构信息

Dept. of Endodontics, Stomatological Hospital of Luzhou Medical College, Luzhou 646000, China.

出版信息

Hua Xi Kou Qiang Yi Xue Za Zhi. 2010 Jun;28(3):306-10.

PMID:20635664

Abstract

OBJECTIVE

To explore the feasibility of methyl thiazolyl tetrazolium (MTT) colorimetric method and the applied condition for the normal bacteria in the mouth, as Streptococcus mutans (S. mutans), Streptococcus sanguis (S. sanguis), Haemophilus actinomycetemcomitans (H. actinomycetemcomitans).

METHODS

Colony forming units (CFU) which was the standard antitheses was used to count bacteria. This study would gain some parameters by changing wavelength, reactive time, dosage and so on. MTT colorimetric method was applied in the counting of S. mutans, S. sanguis and H. actinomycetemcomitans.

RESULTS

When counting S. mutans, the best wavelength was 510 nm, the best range was 1.5 x 10(5) - 1.0 x 10(7) CFU x mL(-1). When counting S. sanguis, the best wavelength was 545 nm, the best range was 1.5 x 10(5) - 2.0 x 10(7) CFU x mL(-1). When counting H. actinomycetemcomitans, the best wavelength was 557 nm, the best range was 1.0 x 10(6) - 5.0 x 10(7) CFU x mL(-1). MTT colorimetric method can be used for different aged S. mutans, S. sanguis and H. actinomycetemcomitans.

CONCLUSION

Oral bacteria could be counted by MTT colorimetric method, which is fast and convenient.

摘要

目的

探讨噻唑蓝（MTT）比色法用于口腔常见细菌如变形链球菌、血链球菌、伴放线放线杆菌计数的可行性及应用条件。

方法

以菌落形成单位（CFU）为标准对照进行细菌计数，通过改变波长、反应时间、加样量等获取相关参数，应用MTT比色法对变形链球菌、血链球菌和伴放线放线杆菌进行计数。

结果

计数变形链球菌时，最佳波长为510nm，最佳测定范围为1.5×10⁵ - 1.0×10⁷CFU·mL⁻¹；计数血链球菌时，最佳波长为545nm，最佳测定范围为1.5×10⁵ - 2.0×10⁷CFU·mL⁻¹；计数伴放线放线杆菌时，最佳波长为557nm，最佳测定范围为1.0×10⁶ - 5.0×10⁷CFU·mL⁻¹。MTT比色法可用于不同菌龄的变形链球菌、血链球菌和伴放线放线杆菌计数。