Zhang Gui-li, Zeng Tao, Wang Qing-shan, Zhao Xiu-lan, Song Fu-yong, Xie Ke-qin
Institute of Toxicology, Shandong University,Jinan 250012, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2010 Mar;28(3):190-4.
To observe and compare the protective effect of garlic oil against carbon tetrachloride (CCL)-induced acute liver injury.
The experiments include 4 preventive groups and 2 therapeutic groups. In every preventive and therapeutic group, the mice were randomized into 6 groups with 15 each, including one negative control group, one solvent control group, one CCl4 model group and 3 garlic oil groups (25, 50, and 100 mg/kg body weight). Before given a single gavage of CCl4 (80 mg/kg), the mice were pretreated with garlic oil by gavage in preventive group 1 (30 days, once daily), preventive group 2 (5 days, once daily), preventive group 3 (ahead of 2 h, once), preventive group 4 (immediately, once) or the vehicle (corn oil, 10 ml/kg) in solvent control group. In therapeutic groups, the mice were gavaged garlic oil 2 h (once, in therapeutic 1) or for 5 days (once daily, in therapeutic 2) after administration CCl. After 24 h of the last administration, blood was collected and centrifuged at 2500 r/min at 4 degrees C for 10 min, and serum was removed to measure ALT and AST activities. The liver was dissected, weighed to calculate the liver coefficient (relative liver weight). At the same time, the liver samples were studied by histological examinations.
Compared with negative group, the liver coefficient and the activities of ALT and AST in serum of model group were increased remarkably (P < 0.01). Compared with CCl model group, the liver coefficient and the activities of ALT and AST in serum were decreased significantly (P < 0.01) by garlic oil dose-dependently in each preventive group. Simultaneously, histological assessment showed that garlic oil effectively alleviated hepatocyte injuries induced by CCl4. Comparing the preventive effects of garlic oil in every group, it was better in preventive group 3 than others. However, all indexes and histological examinations in therapeutic group 1 did not show the difference with those of CCl4 model group. In therapeutic group 2, all indexes recovered after 5 d of CCl4 administration.
Garlic oil can prevent acute liver injury induced by CCl4 and the effect is better in ahead of 2 h group than others.
观察并比较大蒜油对四氯化碳（CCl）诱导的急性肝损伤的保护作用。
实验包括4个预防组和2个治疗组。在每个预防组和治疗组中，将小鼠随机分为6组，每组15只，包括1个阴性对照组、1个溶剂对照组、1个CCl4模型组和3个大蒜油组（25、50和100mg/kg体重）。在单次灌胃CCl4（80mg/kg）前，预防组1（30天，每日1次）、预防组2（5天，每日1次）、预防组3（提前2小时，1次）、预防组4（立即，1次）的小鼠通过灌胃给予大蒜油，溶剂对照组的小鼠给予赋形剂（玉米油，10ml/kg）。在治疗组中，小鼠在给予CCl后2小时（治疗组1，1次）或5天（治疗组2，每日1次）灌胃大蒜油。在最后一次给药24小时后，采集血液，在4℃下以2500r/min离心10分钟，分离血清以测定ALT和AST活性。解剖肝脏，称重以计算肝脏系数（相对肝脏重量）。同时，对肝脏样本进行组织学检查。
与阴性组相比，模型组的肝脏系数以及血清中ALT和AST活性显著升高（P<0.01）。与CCl模型组相比，各预防组中大蒜油均剂量依赖性地显著降低了肝脏系数以及血清中ALT和AST活性（P<0.01）。同时，组织学评估显示大蒜油有效减轻了CCl4诱导的肝细胞损伤。比较各组大蒜油的预防效果，预防组3的效果优于其他组。然而，治疗组1的所有指标和组织学检查与CCl4模型组相比均未显示出差异。在治疗组2中，CCl4给药5天后所有指标均恢复。
大蒜油可预防CCl4诱导的急性肝损伤，且提前2小时给药组的效果优于其他组。
