Avian Diseases Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan, Sichuan, China.
Virol J. 2010 Jul 17;7:162. doi: 10.1186/1743-422X-7-162.
Knowledge of the intracellular location of a protein can provide useful insights into its function. Bioinformatic studies have predicted that the DEV pUL38 mainly targets the cytoplasm and nucleus. In this study, we obtained anti-pUL38 polyclonal sera. These antibodies were functional in western blotting and immunofluorescence in DEV-infected duck embryo fibroblasts (DEFs). pUL38 was expressed as a 51-kDa protein from 8 h post-infection onward, initially showing a diffuse distribution throughout the cytoplasm, and later in the nucleus. Furthermore, pUL38 was found in purified virus. These results provide the first evidence of the kinetics of expression and intracellular localization of DEV pUL38.
蛋白质的细胞内位置的知识可以为其功能提供有用的见解。生物信息学研究预测，DEV pUL38 主要靶向细胞质和细胞核。在这项研究中，我们获得了抗 pUL38 多克隆血清。这些抗体在 DEV 感染的鸭胚成纤维细胞（DEFs）中的 western blot 和免疫荧光中具有功能。pUL38 在感染后 8 小时开始作为 51kDa 的蛋白表达，最初在细胞质中呈弥散分布，随后在细胞核中分布。此外，pUL38 存在于纯化的病毒中。这些结果首次提供了 DEV pUL38 的表达和细胞内定位动力学的证据。
