Adnan Menderes University, Institute of Health Sciences, Department of Biophysics, Aydin, Turkey.
J Neurosci Methods. 2010 Aug 30;191(2):277-82. doi: 10.1016/j.jneumeth.2010.07.008. Epub 2010 Jul 14.
Electrophysiological and theoretical studies have been performed to investigate peripheral nerve injuries and nerve regeneration. The aim of this work is to evaluate the effect of melatonin functionally and electrophysiologically on peripheral nerve crush injury. Adult male Wistar rats (n=32, 200+/-50 g) were randomly allocated into four groups. Sciatic crush was constituted on left sciatic nerves. Treatment groups received intraperitoneal melatonin at doses of 5 and 20mg/kg for 21 days. Functional nerve recovery was evaluated using sciatic functional index (SFI) every week during the experiment. In vivo electrophysiological measurements were performed at the end of the treatment. The electrophysiological data were also analyzed by wavelet analysis. Melatonin treatments increased the SFI values in the injured sciatic nerves. In vivo electrophysiological measurements showed that melatonin increased the conduction velocities and also decreased the latency values. The wavelet analysis showed that melatonin treatment reduced the densities of high frequency components of compound muscle action potential (CMAP). These results suggest that melatonin application is a promising strategy for the treatment of peripheral nerve crush injuries. Furthermore, analysis of EMG data with wavelet methods seems to give more reliable results to evaluate the nerve recovery.
已进行电生理学和理论研究，以调查周围神经损伤和神经再生。本工作的目的是从功能和电生理学方面评估褪黑素对周围神经挤压伤的作用。雄性 Wistar 大鼠（n=32，200+/-50g）被随机分为四组。左侧坐骨神经受压。治疗组腹腔内给予褪黑素 5 和 20mg/kg，共 21 天。实验过程中每周通过坐骨神经功能指数（SFI）评估神经功能恢复情况。在治疗结束时进行体内电生理测量。电生理数据也通过小波分析进行分析。褪黑素治疗可增加损伤坐骨神经的 SFI 值。体内电生理测量显示褪黑素可增加传导速度并降低潜伏期值。小波分析表明，褪黑素治疗可降低复合肌肉动作电位（CMAP）的高频成分密度。这些结果表明褪黑素的应用是治疗周围神经挤压伤的一种有前途的策略。此外，用小波方法分析肌电图数据似乎可以提供更可靠的结果来评估神经恢复情况。
