College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
Vaccine. 2010 Aug 23;28(37):6006-13. doi: 10.1016/j.vaccine.2010.06.103. Epub 2010 Jul 14.
The in vivo immunoadjuvant effects of CpG oligodeoxynucleotides (CpG-ODN) have been studied extensively in mice and relatively fewer studies have been done in piglets. But so far, the innate immunostimulatory effects of CpG-ODN combination with innate defense-regulator peptides (IDRs) have not been demonstrated. The purpose of this study is to determine the potential effects of CpG-ODN with IDR in newborn piglets. The immunostimulatory abilities of four selected IDRs were compared, among them HH2 showed best immunostimulatory effects in newborn piglets. Hereafter, the abilities of CpG-ODN combined with HH2 to enhance innate immune responses were examined in newborn piglets. The complex of HH2 and CpG-ODN could induce much stronger Th1 cytokine and chemokine responses than HH2 or CpG-ODN alone. HH2-CpG-ODN immunized piglets showed higher B cell percentage in PBMCs than CpG-ODN alone. These in vivo data demonstrated for the first time that subcutaneously (SC) administration of CpG-ODN combined with HH2 is efficient to stimulate innate immune system in newborn piglets.
CpG 寡脱氧核苷酸（CpG-ODN）的体内免疫佐剂作用在小鼠中已得到广泛研究，而在仔猪中进行的研究相对较少。但到目前为止，CpG-ODN 与先天免疫调节剂肽（IDR）的组合的先天免疫刺激作用尚未得到证实。本研究旨在确定 CpG-ODN 与 IDR 在新生仔猪中的潜在作用。比较了四种选定的 IDR 的免疫刺激能力，其中 HH2 在新生仔猪中表现出最佳的免疫刺激作用。此后，研究了 CpG-ODN 与 HH2 联合增强先天免疫应答的能力。HH2 和 CpG-ODN 的复合物比单独的 HH2 或 CpG-ODN 能诱导更强的 Th1 细胞因子和趋化因子反应。HH2-CpG-ODN 免疫的仔猪在 PBMC 中的 B 细胞百分比高于单独使用 CpG-ODN。这些体内数据首次表明，CpG-ODN 与 HH2 联合皮下（SC）给药可有效刺激新生仔猪的先天免疫系统。
