College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
Int Immunopharmacol. 2012 Feb;12(2):415-24. doi: 10.1016/j.intimp.2011.12.015. Epub 2012 Jan 4.
The in vivo immunoadjuvant effects of the combination of CpG oligodeoxynucleotide (CpG ODN) and innate defense-regulator peptides (IDRs) have been studied in mice. However, little is known about the efficacy of these molecules in stimulating the innate intestinal immune system in neonatal piglets. In this study, we observed that intranasal (IN) administration of CpG-IDR (peptide HH2 (VQLRIRVAVIRA)) complex significantly increased intestinal mRNA expression of Th1 cytokines, CC chemokines and CXC chemokines when compared to HH2 and CpG ODN alone. Also an obvious cellular infiltration was observed in the intestine of CpG-HH2-treated neonatal piglets, which was associated with increased protection against Enterotoxigenic Escherichia coli (ETEC). Moreover, we showed that pro-inflammatory cytokine TNF-α was inhibited when CpG ODN combined with HH2. This was the first report that deciphered the role played by CpG-HH2 complex in the intestine of neonatal piglets. This work clearly demonstrated that efficiency of the IN route inducing intestinal responses in neonatal piglets might be taken into consideration for further vaccine development against neonatal intestinal diseases.
已在小鼠中研究了 CpG 寡脱氧核苷酸（CpG ODN）和先天免疫调节剂肽（IDR）联合的体内免疫佐剂效应。然而，对于这些分子在刺激新生仔猪固有肠道免疫系统方面的功效知之甚少。在这项研究中，我们观察到与单独使用 HH2 和 CpG ODN 相比，鼻内（IN）给予 CpG-IDR（肽 HH2（VQLRIRVAVIRA））复合物可显著增加肠道 Th1 细胞因子、CC 趋化因子和 CXC 趋化因子的 mRNA 表达。在接受 CpG-HH2 治疗的新生仔猪的肠道中也观察到明显的细胞浸润，这与对肠毒素性大肠杆菌（ETEC）的保护作用增加有关。此外，我们还表明，CpG ODN 与 HH2 结合时可抑制促炎细胞因子 TNF-α。这是首次报道 CpG-HH2 复合物在新生仔猪肠道中发挥作用。这项工作清楚地表明，IN 途径诱导新生仔猪肠道反应的效率可能需要考虑用于针对新生儿肠道疾病的进一步疫苗开发。
