Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS 39762, USA.
Reprod Biomed Online. 2010 Sep;21(3):312-24. doi: 10.1016/j.rbmo.2010.06.022. Epub 2010 Jun 20.
Spermatozoa deliver more than the paternal genome into the oocyte; they also carry remnant messenger RNA from spermatogenesis. The RNA profiles of spermatozoa from high-fertility and a low-fertility Holstein bulls were analysed using Affymetrix bovine genechips. A total of 415 transcripts out of approximately 24,000 were differentially detected in spermatozoa collected from both bulls (fold change > or =2.0; P<0.01). These transcripts were associated with different cellular functions and biological processes. Spermatozoa from high-fertility bulls contained higher concentrations of transcripts for membrane and extracellular space protein locations, while spermatozoa from the low-fertility bulls were deficient of transcripts for transcriptional and translational factors. Quantitative real-time PCR was used on three low-fertility and four high-fertility bulls to validate the microarray data. Two highly represented transcripts in the microarray analysis (protamine 1 and casein beta 2) were validated, as well as a third transcript (thrombospondin receptor CD36 molecule) that showed a lower concentration in low-fertility bulls. This study presents the global analysis of spermatozoa originating from bulls with opposite fertility. These results provide some specific transcripts in spermatozoa that could be associated with bull fertility.
精子不仅将父系基因组传递给卵子，还携带精子发生过程中残留的信使 RNA。使用 Affymetrix 牛基因芯片分析了来自高产荷斯坦公牛和低产荷斯坦公牛的精子的 RNA 图谱。在从这两头公牛收集的精子中，总共检测到了约 24000 个转录本中的 415 个转录本存在差异（倍数变化>或=2.0；P<0.01）。这些转录本与不同的细胞功能和生物过程有关。高产公牛的精子中含有更高浓度的膜和细胞外空间蛋白位置的转录本，而低产公牛的精子中缺乏转录因子和翻译因子的转录本。使用实时定量 PCR 对 3 头低产公牛和 4 头高产公牛进行验证，以验证微阵列数据。对微阵列分析中两个高度代表性的转录本（鱼精蛋白 1 和酪蛋白 β2）进行了验证，以及第三个转录本（血小板反应蛋白 CD36 分子）在低产公牛中的浓度较低。这项研究对来自具有相反生育力的公牛的精子进行了全面分析。这些结果提供了一些与公牛生育力相关的精子中特定的转录本。
