Barth Albert, Perry Viv E A, Hamilton Lauren E, Sutovsky Peter, Oko Richard
Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
Queensland Sperm Morphology Laboratory (QSML), Goondiwindi, QLD, Australia.
Adv Anat Embryol Cell Biol. 2025;240:297-329. doi: 10.1007/978-3-031-70126-9_7.
New and emerging technologies allow for a deeper and more comprehensive understanding of sperm physiology that can be harnessed to improve bull fertility selection. This chapter focuses on (1) the use of conventional and emerging flow cytometry techniques to further enhance functional sperm assessments; (2) new developments in proteomic and metabolomic biomarkers of bull fertility and how they can better inform fertility evaluations; and (3) the use of sperm selection technologies to optimize the fertility outcomes of bulls in artificial insemination service. As our knowledge of sperm physiology continues to expand, technology will allow for a faster translational capacity and continuous development of techniques. The technologies and techniques presented are current tools that can be used to enhance the efficiency, precision and accuracy of bull fertility assessments and better inform herd management.
新出现的技术使人们能够更深入、更全面地了解精子生理学,从而可用于改进公牛生育力的选择。本章重点关注:(1)使用传统和新兴的流式细胞术技术进一步加强对精子功能的评估;(2)公牛生育力的蛋白质组学和代谢组学生物标志物的新进展,以及它们如何能更好地为生育力评估提供信息;(3)使用精子选择技术优化人工授精服务中公牛的生育结果。随着我们对精子生理学的认识不断扩展,技术将实现更快的转化能力和技术的持续发展。所介绍的技术和方法是当前可用于提高公牛生育力评估的效率、精度和准确性,并更好地为畜群管理提供信息的工具。