Bacteriology Division, National Institute for Biological Standards and Control, Health Protection Agency, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK.
Vaccine. 2010 Aug 31;28(38):6228-34. doi: 10.1016/j.vaccine.2010.07.005. Epub 2010 Jul 16.
The immunogenicity, structure and stability of a combined conjugate vaccine against Haemophilus influenzae type b and meningococcal serogroup C (Hib/MenC) were investigated. A rat model for immunogenicity showed that antibody responses to Hib and MenC in the combined vaccine were similar to or higher than those of individual conjugates given alone, or concomitantly at separate sites. At elevated temperatures, the combination vaccine was slightly more stable than a monovalent Hib-TT vaccine, with respect to molecular size, which could be attributed to differences in the formulations. Following 5 weeks incubation at 56 degrees C, there was some dissociation of high molecular weight conjugate without significant loss of saccharide integrity; however, this did not significantly affect the vaccine immunogenicity, demonstrating the stability of this lyophilized vaccine.
研究了一种针对乙型流感嗜血杆菌和脑膜炎奈瑟球菌 C 群（Hib/MenC）的联合结合疫苗的免疫原性、结构和稳定性。一种用于免疫原性的大鼠模型表明，该联合疫苗对 Hib 和 MenC 的抗体反应与单独给予的单价结合物或同时在不同部位给予的单价结合物相似或更高。在较高温度下，与单价 Hib-TT 疫苗相比，该联合疫苗在分子大小方面稍微更稳定，这可能归因于配方的差异。在 56°C 孵育 5 周后，虽然没有显著损失糖的完整性，但仍有一些高分子量结合物发生解离；然而，这并没有显著影响疫苗的免疫原性，证明了这种冻干疫苗的稳定性。
