Orlin J R, Osen K K, Hovig T
Division of Experimental Neurosurgery, National Hospital, Oslo, Norway.
Anat Rec. 1991 May;230(1):22-37. doi: 10.1002/ar.1092300104.
The dura-arachnoid junction is examined in normal animals and in animals subjected to subdural infusion of blood immediately prior to death, simulating acute subdural hemorrhages. The Norwegian landrace pig is used as the experimental animal. Horseradish peroxidase (HRP) has been added to the injected blood to serve as a macromolecular tracer. The material is studied by light and electron microscopy. Special attention is given to the level of the induced subdural cleavage plane, the total distribution of the infused blood, and the natural sites of drainage. The dura-arachnoid junction, identified here as the subdural compartment (the dural border layer of others), consists of an avascular tissue with flake-like, relatively electron-lucent cells stacked upon each other in several layers with narrow intercellular clefts. Under normal conditions there is no evidence of a so-called "subdural space." When under the present experimental conditions bleeding takes place into this cellular tissue, it splits without any particular, predestined cleavage plane, although most often close to the fibrous matter of the dura. The bleeding extends throughout the cerebral and spinal parts of the compartment and also along the spinal nerve roots. Contamination of the subarachnoid space occurs only in some cases subjected to large infusions and apparently only at spinal levels. The HRP diffuses into the dura, but does not traverse the arachnoid barrier layer.
在正常动物以及在濒死时立即接受硬脑膜下血液输注以模拟急性硬脑膜下出血的动物中，对硬脑膜 - 蛛网膜交界处进行检查。选用挪威长白猪作为实验动物。已将辣根过氧化物酶（HRP）添加到注入的血液中作为大分子示踪剂。通过光学显微镜和电子显微镜对材料进行研究。特别关注诱导的硬脑膜下裂解平面的水平、注入血液的总体分布以及自然引流部位。此处确定的硬脑膜 - 蛛网膜交界处为硬脑膜下腔隙（其他人所说的硬脑膜边界层），由无血管组织构成，其中片状、相对电子密度较低的细胞相互堆叠成多层，细胞间裂隙狭窄。在正常情况下，没有所谓“硬脑膜下间隙”的证据。在当前实验条件下，当血液流入这种细胞组织时，它会分裂，但没有任何特定的、预定的裂解平面，不过最常靠近硬脑膜的纤维物质处。出血会扩展到该腔隙的脑和脊髓部分，也会沿着脊神经根蔓延。蛛网膜下腔隙仅在一些接受大量输注的情况下发生污染，且显然仅在脊髓水平。HRP会扩散到硬脑膜中，但不会穿过蛛网膜屏障层。
