School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, Singapore, Singapore.
Appl Biochem Biotechnol. 2011 Jan;163(2):235-46. doi: 10.1007/s12010-010-9033-x. Epub 2010 Jul 18.
Horticultural waste collected from a landscape company in Singapore was utilized as the substrate for the production of laccase under solid-state fermentation by Trametes versicolor. The effects of substrate particle size, types of inducers, incubation temperature and time, initial medium pH value, and moisture content on laccase production were investigated. The optimum productivity of laccase (8.6 U/g substrate) was achieved by employing horticultural waste of particle size greater than 500 μm and using veratryl alcohol as the inducer. The culture was at 30 °C for 7 days at moisture content of solid substrate of 85% and initial pH 7.0. The decolorization was also investigated in order to assess the degrading capability of the ligninolytic laccase obtained in the above-mentioned cultures. The decolorization degree of a model dye, phenol red, was around 41.79% in 72 h of incubation. By far, this is the first report on the optimization of laccase production by T. versicolor under solid-state fermentation using horticultural waste as the substrate.
从新加坡的一家园林公司收集的园艺废物被用作固态发酵中，彩绒革盖菌生产漆酶的基质。研究了基质粒径、诱导剂种类、培养温度和时间、初始培养基 pH 值和含水量对漆酶生产的影响。采用粒径大于 500μm 的园艺废物，以藜芦醇为诱导剂，可获得漆酶（8.6 U/g 基质）的最佳产量。在 30°C 下，固体基质含水量为 85%，初始 pH 值为 7.0，培养 7 天。还研究了脱色情况，以评估上述培养物中获得的木质素降解漆酶的降解能力。在 72 小时的孵育中，模型染料苯酚红的脱色率约为 41.79%。到目前为止，这是首次报道使用园艺废物作为基质，通过固态发酵优化彩绒革盖菌生产漆酶。
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