Faculty of Food Technology Osijek, J. J. Strossmayer University of Osijek, F. Kuhača 18, Osijek, Croatia.
Appl Biochem Biotechnol. 2012 Jan;166(1):36-46. doi: 10.1007/s12010-011-9401-1. Epub 2011 Oct 12.
Laccases are very interesting biocatalysts for several industrial applications. Its production by different white-rot fungi can be stimulated by a variety of inducing substrates, and the use of lignocellulosic wastes or industrial by-products is one of the possible approaches to reduce production costs. In this work, various industrial wastes were tested for laccase production by Trametes versicolor MZKI G-99. Solid waste from chemomechanical treatment facility of a paper manufacturing plant showed the highest potential for laccase production. Enzyme production during submerged cultivation of T. versicolor on the chosen industrial waste has been further improved by medium optimization using genetic algorithm. Concentrations of five components in the medium were optimized within 60 shake-flasks experiments, where the highest laccase activity of 2,378 U dm(-3) was achieved. Waste from the paper industry containing microparticles of CaCO(3) was found to stimulate the formation of freely dispersed mycelium and laccase production during submerged cultivation of T. versicolor. It was proven to be a safe and inexpensive substrate for commercial production of laccase and might be more widely applicable for metabolite production by filamentous fungi.
漆酶是一类非常有趣的生物催化剂，在许多工业应用中都有重要作用。不同白腐真菌产生漆酶的过程可以被多种诱导底物所刺激，而利用木质纤维素废弃物或工业副产物是降低生产成本的可能途径之一。在这项工作中，使用变色栓菌 MZKI G-99 对各种工业废物进行了产漆酶测试。造纸厂化机浆处理设施的固体废料显示出了最高的产漆酶潜力。通过使用遗传算法对选择的工业废料进行液体深层发酵中的培养基优化，进一步提高了酶的生产。在 60 次摇瓶实验中优化了培养基中 5 种成分的浓度，获得了最高的漆酶活性 2,378 U dm(-3)。含有碳酸钙微粒的造纸工业废料被发现可以刺激变色栓菌液体深层发酵中游离分散菌丝体和漆酶的形成。它被证明是一种安全且廉价的商业漆酶生产用底物，可能更广泛地适用于丝状真菌的代谢产物生产。
