Deresz L F, Sprinz E, Kramer A S, Cunha G, de Oliveira A R, Sporleder H, de Freitas D R J, Lazzarotto A R, Dall'Ago P
Cardiologia e Ciencias Cardiovasculares da FAMED/UFRGS, Porto Alegre, Rio Grande do Sul, Brasil.
AIDS Care. 2010 Nov;22(11):1410-7. doi: 10.1080/09540121003758549.
Human immunodeficiency virus (HIV)-infected subjects have increased levels of oxidative stress which could impair immunological function and therefore contribute to the progression of AIDS. These characteristics are usually evaluated at rest and responses to exercise have yet to be evaluated. The aim of the present study was to assess the effect of a bout of aerobic exercise followed by resistance exercises on antioxidant system in HIV-infected and non-HIV subjects. There were included 14 cases (HIV-positive) and 14 controls (HIV-negative). The exercise protocol consisted of a single session of 20 minutes on a cycloergometer followed by a set of six resistance exercises. The activity of glutathione S-transferase (GST) and catalase were measured in plasma samples, total glutathione (TGSH) and thiobarbituric acid reactive substances (TBARS) were measured in erythrocytes. T CD4+ cells, T CD8+, viral load, complete blood count, and white blood count were also assessed. All measurements were performed at three times: baseline, after aerobic exercise, and after resistance exercises. At baseline, the HIV group had lower GST activity than controls, but after the exercise session GST values were similar in both groups. Compared to the control group TGSH was significantly lower in the HIV group at baseline, after aerobic and resistance exercises. The control group presented higher TBARS values after aerobic exercise compared to the HIV group. The neutrophil count was lower in the HIV group after aerobic and resistance exercises. These data indicate that HIV-infected subjects had lower antioxidant activity at rest. Physical exercise stimulated the enzymatic activity similarly in both groups.
感染人类免疫缺陷病毒（HIV）的受试者氧化应激水平升高，这可能损害免疫功能，从而导致艾滋病的进展。这些特征通常在静息状态下评估，而运动反应尚未得到评估。本研究的目的是评估一轮有氧运动后接着进行抗阻运动对HIV感染和未感染HIV受试者抗氧化系统的影响。研究纳入了14例（HIV阳性）和14例对照（HIV阴性）。运动方案包括在自行车测力计上进行20分钟的单次运动，随后进行一组六项抗阻运动。在血浆样本中测量谷胱甘肽S-转移酶（GST）和过氧化氢酶的活性，在红细胞中测量总谷胱甘肽（TGSH）和硫代巴比妥酸反应性物质（TBARS）。还评估了T CD4+细胞、T CD8+、病毒载量、全血细胞计数和白细胞计数。所有测量均在三个时间点进行：基线、有氧运动后和抗阻运动后。在基线时，HIV组的GST活性低于对照组，但运动后两组的GST值相似。与对照组相比，HIV组在基线、有氧运动和抗阻运动后的TGSH显著较低。与HIV组相比，对照组在有氧运动后的TBARS值更高。HIV组在有氧运动和抗阻运动后的中性粒细胞计数较低。这些数据表明，HIV感染的受试者在静息状态下抗氧化活性较低。体育锻炼在两组中同样刺激了酶活性。
