Verschraegen C F, Giovanella B C, Mendoza J T, Kozielski A J, Stehlin J S
St. Joseph Hospital Cancer Research Laboratory, Houston, Texas 77003.
Anticancer Res. 1991 Mar-Apr;11(2):529-35.
The purpose of this study was to examine whether organ specific metastasis can be produced by different subpopulations of cells isolated from a single human melanoma. Three clones, SB1, SB2, SB3, and a variant line SB1 Asc (obtained from ascites fluid after i.v. inoculation of SB1 cells in a nude mouse) - all isolated from a primary human cutaneous melanoma - were inoculated into the left ventricle (i.c.) or intravenously (i.v.) into nude mice. Three to 16 weeks later, the animals were killed and autopsied. The clones produced different patterns of metastasis. Three to 4 weeks after i.c. inoculation of 2 x 10(6) cells, cells of clone SB1 produced numerous rapidly progressing metastases in the brain, whereas cells of SB1 Asc produced large metastases in the adrenals and sometimes in the ovaries. Cells of clones SB2 and SB3 produced a few small metastases without any characteristics pattern. The initial arrest of tumor cells in various organs did not predict the outcome of metastasis. This conclusion is based on studies of distribution and fate of tumor cells labeled with 111Indium Oxine. Therefore, organ specific metastasis produced by human melanoma cells inoculated i.c. into nude mice is likely to depend on the interaction of unique metastatic cells with organ environment.
本研究的目的是检验从单一人类黑色素瘤分离出的不同细胞亚群是否能产生器官特异性转移。三个克隆株SB1、SB2、SB3，以及一个变异株SB1 Asc（通过将SB1细胞静脉注射到裸鼠体内后从腹水获得）——均从原发性人类皮肤黑色素瘤中分离——被接种到裸鼠的左心室（颅内）或静脉内（静脉注射）。三到十六周后，处死动物并进行尸检。这些克隆株产生了不同的转移模式。在颅内接种2×10⁶个细胞三到四周后，克隆株SB1的细胞在脑内产生了大量迅速进展的转移灶，而SB1 Asc的细胞在肾上腺产生了大的转移灶，有时也在卵巢产生转移灶。克隆株SB2和SB3的细胞产生了一些小的转移灶，没有任何特征性模式。肿瘤细胞在各个器官的初始滞留并不能预测转移的结果。这一结论基于对用¹¹¹铟氧喹啉标记的肿瘤细胞的分布和命运的研究。因此，颅内接种到裸鼠体内的人类黑色素瘤细胞产生的器官特异性转移可能取决于独特的转移细胞与器官环境的相互作用。
