National Institute of Health and Nutrition, Shinjuku-ku, Tokyo, Japan.
Biochem Biophys Res Commun. 2010 Aug 13;399(1):98-103. doi: 10.1016/j.bbrc.2010.07.048. Epub 2010 Jul 17.
Subjects with high blood levels of inflammatory markers and patients with chronic inflammatory disorders are at high risk for stroke. Dietary restriction (DR) suppresses systemic inflammation to deter age-related chronic diseases. To examine whether DR delays the onset of stroke, 10-week-old stroke-prone spontaneously hypertensive rats (SHRSP) were assigned to either a control (ad libitum) or DR (50% diet of control) group, and day of stroke onset and lifespan were observed. DR markedly delayed the onset of stroke in SHRSP compared to control without affecting blood pressure. Day of stroke onset (median) in the control group was 34days, whereas it was 70days in the DR group. After 2weeks of DR and before the onset of stroke, plasma levels of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), and monocyte chemoattractant protein-1 (MCP-1) and their mRNA expression levels in adipose tissue were significantly lower in the DR rats than in the control rats. Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mRNA expression levels in cerebrovascular endothelial cells (CVECs), and macrophage infiltration into brain were lower in the DR rats than in the control rats. IL-1beta and TNF-alpha treatment in CVECs increased MCP-1, C-reactive protein, ICAM-1, and VCAM-1 mRNA and their protein levels in vitro. In conclusion, suppression of inflammation in response to DR may lead to a delay in the onset of stroke independent of any effect on blood pressure in SHRSP.
血液中炎症标志物水平高的受试者和患有慢性炎症性疾病的患者发生中风的风险很高。饮食限制（DR）可抑制全身炎症，防止与年龄相关的慢性疾病。为了研究 DR 是否会延迟中风的发作，将易发生中风的自发性高血压大鼠（SHRSP）分配到对照组（自由进食）或 DR 组（对照组饮食的 50%），观察中风发作的日期和寿命。与对照组相比，DR 明显延迟了 SHRSP 中风的发作，而不影响血压。对照组中风发作的日期（中位数）为 34 天，而 DR 组为 70 天。在 DR 后 2 周且在中风发作之前，DR 大鼠的血浆白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和单核细胞趋化蛋白-1（MCP-1）水平及其在脂肪组织中的 mRNA 表达水平明显低于对照组大鼠。DR 大鼠脑血管内皮细胞（CVEC）中的细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）mRNA 表达水平以及巨噬细胞浸润到大脑中的水平均低于对照组大鼠。IL-1β和 TNF-α在 CVEC 中的处理增加了 MCP-1、C 反应蛋白、ICAM-1 和 VCAM-1 mRNA 及其蛋白水平。总之，DR 引起的炎症抑制可能导致 SHRSP 中风发作延迟，而与血压无任何影响。
