Chiba Tsuyoshi, Yokotani Kaori, Suzuki Sachina, Itoh Tatsuki, Umegaki Keizo
Information Center, National Institute of Health and Nutrition.
J Atheroscler Thromb. 2015;22(9):958-70. doi: 10.5551/jat.27789. Epub 2015 Apr 7.
Resveratrol has been shown to mimic the beneficial effects of dietary restriction (DR). We previously reported that DR delays stroke onset and extends the lifespan in Stroke-Prone Spontaneously Hypertensive rats (SHRSP). Therefore, we examined whether resveratrol mimics DR and delays stroke onset in SHRSP.
Cerebrovascular endothelial cells (CVECs) from SHRSP were treated with resveratrol, and the inflammatory gene expression levels and NFκB protein levels were measured. In order to address the effects of resveratrol in vivo, SHRSP (male, 10 weeks of age) were fed an experimental diet containing several doses of resveratrol (0 - 0.05% (w/w)), after which we measured the plasma cytokine levels and examined the stroke onset and lifespan.
Treatment with resveratrol (100 μM, 24 hours) in CVECs from SHRSP significantly decreased the interleukin (IL)-1β-induced monocyte chemoattractant protein-1 (MCP-1) mRNA expression levels and p50 and p65 protein levels in the nuclear fraction. When the SHRSP were fed a diet containing resveratrol for one week, the resveratrol treatment did not affect the plasma lipid and glucose levels, body weight or weight of each tissue. Resveratrol slightly, but not significantly, decreased the plasma levels of IL-1β and MCP-1 compared with that observed in the control group. In addition, resveratrol decreased the IL-1β and MCP-1 mRNA expression levels in the brain versus the control animals. However, no doses of resveratrol delayed stroke onset or extended the lifespan in SHRSP.
In this study, resveratrol did not delay stroke onset in SHRSP, although it partially suppressed systemic and cerebral inflammation. These results suggest that resveratrol does not mimic the beneficial effects of DR on stroke in vivo.
白藜芦醇已被证明可模拟饮食限制（DR）的有益作用。我们之前报道过，饮食限制可延迟易中风自发性高血压大鼠（SHRSP）的中风发作并延长其寿命。因此，我们研究了白藜芦醇是否能模拟饮食限制并延迟SHRSP的中风发作。
用白藜芦醇处理SHRSP的脑血管内皮细胞（CVECs），并测量炎症基因表达水平和NFκB蛋白水平。为了研究白藜芦醇在体内的作用，给10周龄雄性SHRSP喂食含几种剂量白藜芦醇（0 - 0.05%（w/w））的实验饮食，之后测量血浆细胞因子水平，并检查中风发作情况和寿命。
用100μM白藜芦醇处理SHRSP的CVECs 24小时，可显著降低白细胞介素（IL）-1β诱导的单核细胞趋化蛋白-1（MCP-1）mRNA表达水平以及核组分中p50和p65蛋白水平。当给SHRSP喂食含白藜芦醇的饮食一周时，白藜芦醇处理对血浆脂质和葡萄糖水平、体重或各组织重量没有影响。与对照组相比，白藜芦醇使血浆IL-1β和MCP-1水平略有降低，但不显著。此外，与对照动物相比，白藜芦醇降低了大脑中IL-1β和MCP-1 mRNA表达水平。然而，任何剂量的白藜芦醇都未延迟SHRSP的中风发作或延长其寿命。
在本研究中，白藜芦醇虽部分抑制全身和脑部炎症，但未延迟SHRSP的中风发作。这些结果表明，白藜芦醇不能模拟饮食限制对体内中风的有益作用。
