Department of Biomedical Engineering, Tel-Aviv University, Israel.
Acta Biomater. 2011 Jan;7(1):325-36. doi: 10.1016/j.actbio.2010.07.013. Epub 2010 Jul 17.
About 70% of all people with severe burns die from related infections, despite advances in treatment regimens and the best efforts of nurses and doctors. Although silver-eluting wound dressings are available for addressing this problem, there is growing evidence of the deleterious effects of such dressings in delaying the healing process owing to cellular toxicity. A new concept of antibiotic-eluting composite wound dressings is described here. These dressings are based on a polyglyconate mesh coated with a porous poly-(dl-lactic-co-glycolic acid) matrix loaded with antibiotic drugs. The effect of antibiotic release on bacterial inhibition was studied, and cell cytotoxicity was examined. The dressings resulted in a 99.99% decrease in the viable counts of Pseudomonas aeruginosa and Staphylococcus albus at very high initial inoculations of 10⁷-10⁸ CFU ml⁻¹ after only 1 day, while such a decrease in Staphylococcus aureus was obtained within 3 days. Bacterial inhibition zones around the dressing material were found to persist for 2 weeks, indicating a long-lasting antimicrobial effect. Despite severe toxicity to bacteria, the dressing material was found to have no toxic effect on cultured fibroblasts, indicating that the new antibiotic-eluting wound dressings represent an effective option for selective treatment of bacterial infections.
尽管治疗方案不断进步，医护人员也竭尽全力，但仍有约 70%的严重烧伤患者死于相关感染。虽然有含银的创伤敷贴可用于解决这个问题，但越来越多的证据表明，这些敷贴会因细胞毒性而延迟愈合过程。本文介绍了一种新型抗生素敷贴的概念。这种敷贴基于聚糖酸网，表面涂有多孔聚（DL-丙交酯-乙交酯）基质，负载抗生素药物。研究了抗生素释放对抑菌效果的影响，并检测了细胞毒性。结果显示，即使在初始接种量为 10⁷-10⁸ CFU ml⁻¹的情况下，仅 1 天后，铜绿假单胞菌和白色葡萄球菌的活菌数就减少了 99.99%，而金黄色葡萄球菌则在 3 天内减少。在敷料周围发现抑菌区持续了 2 周，表明其具有持久的抗菌效果。尽管对细菌有严重毒性，但敷料对培养的成纤维细胞没有毒性作用，表明新型抗生素敷贴是治疗细菌感染的有效选择。
