由B16黑色素瘤细胞和3T3成纤维细胞条件培养基产生的细胞学效应。
Cytological effects of culture media conditioned by B16 melanoma cells and 3T3 fibroblasts.
作者信息
Coucke P H, Siwek B L, De Pauw-Gillet M C, Bassleer R J
机构信息
Department of Histology and Cytology, Faculty of Medicine, University of Liège, Belgium.
出版信息
Anticancer Res. 1991 Mar-Apr;11(2):801-4.
Culture media conditioned (CM) by mixed populations of mouse B16 melanoma cells and 3T3 fibroblasts cultivated as monolayers exert cytological effects on B16 or 3T3 cells when treated separately in culture. By ultrafiltration of these CM, we show that a stimulatory activity on B16 melanoma cells proliferation is present in fractions with M.W. greater than 10,000 daltons. A strong cytotoxic activity for B16 melanoma cells and, to a lower degree, for 3T3 fibroblasts is detected in fractions with M.W. less than 1,000 daltons. The ultrastructural analysis of cells (B16 or 3T3) treated with cytotoxic fractions reveals in them mitochondrial swelling, blebs, broken membranes and dead cells.
单层培养的小鼠B16黑色素瘤细胞和3T3成纤维细胞混合群体所条件化的培养基（CM），在培养中分别处理时，对B16或3T3细胞具有细胞学效应。通过对这些CM进行超滤，我们发现分子量大于10,000道尔顿的组分中存在对B16黑色素瘤细胞增殖的刺激活性。在分子量小于1,000道尔顿的组分中检测到对B16黑色素瘤细胞有强烈的细胞毒性活性，对3T3成纤维细胞的细胞毒性活性较低。用细胞毒性组分处理的细胞（B16或3T3）的超微结构分析显示，它们有线粒体肿胀、泡状突起、膜破裂和死亡细胞。
Zotero 插件