小鼠B16黑色素瘤细胞和3T3成纤维细胞球体中的层粘连蛋白及67kD层粘连蛋白结合蛋白
Laminin and 67 kD laminin binding protein in mouse B16 melanoma cells and 3T3 fibroblast spheroids.
作者信息
Siwek B L, Munaut C, Bonjean K A, Coucke P H, Noel A C, De Pauw-Cillet M C, Foidart J M, Bassleer R J
机构信息
Department of Histology and Cytology, Faculty of Medicine, University of Liége, Belgium.
出版信息
Anticancer Res. 1992 Nov-Dec;12(6B):2011-6.
Multicellular spheroids which promote cell-cell and cell-matrix interactions were prepared in culture with mouse B16 melanoma cells (pigmented or non pigmented) alone or mixed with mouse 3T3 fibroblasts. Their volume and proliferation or necrosis rate were evaluated. As measured by dot blot immunoassay, laminin was mainly produced by fibroblasts rather than by melanoma cells. High levels of laminin B1 chain mRNA were detected only in spheroids composed of 3T3 fibroblasts. The levels of 67 kD laminin binding protein mRNA were high in all cell populations studied here.
通过培养制备促进细胞间和细胞与基质相互作用的多细胞球体，所用细胞为单独的小鼠B16黑色素瘤细胞（有色素或无色素）或与小鼠3T3成纤维细胞混合。评估了它们的体积以及增殖或坏死率。通过斑点印迹免疫测定法检测发现，层粘连蛋白主要由成纤维细胞而非黑色素瘤细胞产生。仅在由3T3成纤维细胞组成的球体中检测到高水平的层粘连蛋白B1链mRNA。在此研究的所有细胞群体中，67 kD层粘连蛋白结合蛋白mRNA的水平都很高。
Zotero 插件