Salle V, Raux H, Souttou B, Israel L, Crépin M
Université de Paris-Nord, Institut d'Oncologie Cellulaire et Moléculaire Humaine (I.O.C.M.H.), Bobigny, France.
Anticancer Res. 1991 Mar-Apr;11(2):895-900.
Primary cultures of non malignant human breast tissues, benign mastopathies and breast carcinoma were performed in defined culture conditions. Epithelial cells from these primary cultures were characterized for mammary epithelial cell specific markers, for in vitro cell proliferation, for steroid receptors and hormone sensitivity (estradiol, progesterone and prolactin) and for EGF sensitivity. We show that although some mastopathies have estradiol and progesterone receptors, they did not respond to hormone treatment. Human prolactin had no effect on the proliferation of one mastopathy but stimulates the cell growth of another fibrocystic mastopathy. EGF was capable of stimulating the three types of primary cultures. As regards growth characteristics, steroid hormone receptors and prolactin sensitivity, phenotypes of mastopathy cells differ from each other; some are similar to non malignant cells, whereas others are comparable to tumor cells.
在特定培养条件下对非恶性人乳腺组织、良性乳腺疾病和乳腺癌进行原代培养。对这些原代培养的上皮细胞进行乳腺上皮细胞特异性标志物、体外细胞增殖、类固醇受体和激素敏感性（雌二醇、孕酮和催乳素）以及表皮生长因子（EGF）敏感性的鉴定。我们发现，尽管一些乳腺疾病有雌二醇和孕酮受体，但它们对激素治疗无反应。人催乳素对一种乳腺疾病的增殖无影响，但能刺激另一种纤维囊性乳腺疾病的细胞生长。EGF能够刺激这三种类型的原代培养物。就生长特性、类固醇激素受体和催乳素敏感性而言，乳腺疾病细胞的表型彼此不同；一些与非恶性细胞相似，而另一些与肿瘤细胞相当。
