Larsson O, Blegen H, Wejde J, Zetterberg A
Department of Tumor Pathology, Karolinska Hospital, Stockholm, Sweden.
Cell Biol Int. 1993 Jun;17(6):565-71. doi: 10.1006/cbir.1993.1100.
The growth regulation of human mammary epithelial cells (HMEC) cultured in a growth factor/hormone-enriched (e.g. EGF, insulin) medium with bovine pituitary extract as the only undefined supplement was studied. The doubling times of the cultures, in which the cells appear in colonies, was 55-72 h, and a considerable intercolonial heterogenecity in proliferative activity could be demonstrated. However, every colony, irrespective of the size of the growth fraction, comprised a sub-population of rapidly growing cells which had a mean generation time of approximately 22 h. When insulin was removed from the culture medium, HMEC proliferation was inhibited. This growth inhibition was shown to be a result of a cell cycle-specific block.
研究了在富含生长因子/激素（如表皮生长因子、胰岛素）的培养基中培养的人乳腺上皮细胞（HMEC）的生长调节，该培养基以牛垂体提取物作为唯一未明确成分的补充剂。细胞呈集落生长的培养物的倍增时间为55 - 72小时，并且可以证明增殖活性存在相当大的集落间异质性。然而，每个集落，无论生长分数大小，都包含一个快速生长的细胞亚群，其平均世代时间约为22小时。当从培养基中去除胰岛素时，HMEC的增殖受到抑制。这种生长抑制被证明是细胞周期特异性阻滞的结果。
