推测检验试剂对使用实时聚合酶链反应的人体血液确认检验和 DNA 分析的影响。
Effect of presumptive tests reagents on human blood confirmatory tests and DNA analysis using real time polymerase chain reaction.
机构信息
Laboratório de Perícias, Instituto Geral de Perícias do Estado do Rio Grande do Sul, Avenida Azenha, 255 CEP 90160-000 Porto Alegre, Rio Grande do Sul, Brazil.
出版信息
Forensic Sci Int. 2011 Mar 20;206(1-3):58-61. doi: 10.1016/j.forsciint.2010.06.017.
Bloodstains often constitute the major physical evidence in crime investigation. Diluted blood invisible to the naked eye can be detected through presumptive tests however such tests can damage samples and prevent further processing such as DNA analysis. In this study, we compared the effects of luminol (prepared according to Weber [15]), Luminol 16(®), Bluestar(®) and benzidine for inhibition in the human antiglobulin test and the human hemoglobin immunochromatographic test and on the total human DNA concentration up to 120 days after sample treatment. Treatment with both luminol solutions and Bluestar(®) still allowed positive results for the immunologic tests, indicating non-interference with human blood confirmatory tests. However, samples treated with benzidine could not be further analyzed by serological tests. Also, DNA quantification showed that 48h after benzidine treatment, but not luminol or Bluestar solution application, sample DNA was degraded. Luminol 16(®) caused DNA degradation already at 30 days post-application. At 120 days post treatment, all samples treated with any of the agents but not untreated samples had DNA degradation.
血痕通常构成犯罪调查中的主要物证。虽然肉眼看不见的稀释血液可以通过预试验检测到，但此类试验可能会损坏样本并阻止进一步处理，如 DNA 分析。在本研究中，我们比较了鲁米诺（根据 Weber [15] 制备）、Luminol 16(®)、Bluestar(®)和联苯胺对人球蛋白试验和人血红蛋白免疫层析试验以及样本处理后 120 天内总人 DNA 浓度的抑制作用。两种鲁米诺溶液和 Bluestar(®)的处理仍允许免疫检测呈阳性，表明不会干扰人血确认检测。然而，用联苯胺处理的样本不能再进行血清学检测。此外，DNA 定量显示，在联苯胺处理后 48 小时，但在鲁米诺或 Bluestar 溶液应用后，样本 DNA 降解。Luminol 16(®)在应用后 30 天就导致 DNA 降解。在治疗后 120 天，所有用任何试剂处理的样本，但未经处理的样本，都有 DNA 降解。
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