łuczak Sylwia, Woźniak Marcin, Papuga Marta, Stopińiska Katarzyna, Sliwka Karol
Katedry Medycyny Sadowej, Collegium Medicum im. Ludwika.
Arch Med Sadowej Kryminol. 2006 Oct-Dec;56(4):239-45.
The objective of the present study was to compare the effectiveness of two chemical agents--Bluestar and luminol--in detection of bloodstains. The experiments were performed to test for bloodstain detection sensitivity, chemical stability and to investigate the effect of both reagents on DNA typing. During this study, the authors prepared serial dilutions (1:2 to 1:10 000 000) of fresh blood, as well as dilutions of 25-year old blood on Whatman 3MM blotting paper. Additional dilutions of fresh blood were spotted on a glass surface. The experiments showed very similar results for both investigated reagents, although the Bluestar solution proved to be more stable (at least 7 days after the preparation) as compared to luminol (stable for not more than 24 hours). Both reagents showed a higher sensitivity for diluted bloodstains on a glass surface than for similar stains on filter paper. The investigators also demonstrated that multiplex amplification of DNA was feasible after Bluestar or luminol treatment, although the detected bloodstains might be too diluted to allow for effective DNA extraction and amplification.
本研究的目的是比较两种化学试剂——蓝星试剂和鲁米诺——在检测血迹方面的有效性。进行实验以测试血迹检测灵敏度、化学稳定性，并研究两种试剂对DNA分型的影响。在本研究中，作者制备了新鲜血液的系列稀释液（1:2至1:10 000 000），以及在沃特曼3MM滤纸上25年陈旧血液的稀释液。新鲜血液的额外稀释液点样在玻璃表面。实验表明，两种研究试剂的结果非常相似，尽管蓝星溶液与鲁米诺相比（稳定不超过24小时）被证明更稳定（制备后至少7天）。两种试剂对玻璃表面稀释血迹的灵敏度均高于滤纸表面类似血迹。研究人员还证明，经蓝星试剂或鲁米诺处理后，DNA的多重扩增是可行的，尽管检测到的血迹可能过于稀释，无法进行有效的DNA提取和扩增。
