皮肤移植成活率、肉芽形成及上皮形成：对髓样细胞缺氧诱导因子1α的依赖性。
Skin graft take rates, granulation, and epithelialization: dependence on myeloid cell hypoxia-inducible factor 1alpha.
作者信息
Vural Emre, Berbée Maaike, Acott Alison, Blagg Ross, Fan Chun-Yang, Hauer-Jensen Martin
机构信息
Department of Otolaryngology-Head and Neck Surgery, University of Arkansas for Medical Sciences, 4301 W Markham, Slot 543, Little Rock, AR 72205, USA.
出版信息
Arch Otolaryngol Head Neck Surg. 2010 Jul;136(7):720-3. doi: 10.1001/archoto.2010.103.
OBJECTIVE
To compare the "take" rates of skin grafts between myeloid-selective hypoxia-inducible factor (HIF) 1alpha knockout (KO) and wild-type (WT) mice. Production of the alpha subunit of HIF-1alpha is increased in healing wounds, which stimulates expression of vascular endothelial growth factor (VEGF) to promote angiogenesis. Therefore, the take rate of skin grafts may be closely associated with the presence or absence of HIF-1alpha production in the recipient bed.
DESIGN
The percentage of healthy graft areas obtained by planimetry and scores for epithelialization and granulation tissue formation obtained by histopathologic analysis were compared in 12 KO and 12 WT mice following skin grafting.
RESULTS
The graft take rate was significantly impaired in the KO group (P = .009), whereas epithelialization (P = .46) or granulation (P = .41) tissue formation scores did not reveal any significant differences.
CONCLUSION
Hypoxia-inducible factor 1alpha in myeloid cells may be an important molecule for revascularization of avascular tissues such as skin grafts, probably owing to its stimulating effect on angiogenesis.
目的
比较骨髓选择性缺氧诱导因子（HIF）1α基因敲除（KO）小鼠和野生型（WT）小鼠皮肤移植的“成活”率。在愈合伤口中，HIF-1α的α亚基生成增加，其可刺激血管内皮生长因子（VEGF）表达以促进血管生成。因此，皮肤移植的成活速率可能与受区中HIF-1α的生成情况密切相关。
设计
在12只基因敲除小鼠和12只野生型小鼠进行皮肤移植后，比较通过平面测量法获得的健康移植区百分比以及通过组织病理学分析获得的上皮化和肉芽组织形成评分。
结果
基因敲除组的移植成活率显著受损（P = 0.009），而上皮化（P = 0.46）或肉芽（P = 0.41）组织形成评分未显示任何显著差异。
结论
骨髓细胞中的缺氧诱导因子1α可能是皮肤移植等无血管组织血管再生的重要分子，这可能归因于其对血管生成的刺激作用。
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