[益气化瘀中药对糖尿病大鼠皮肤溃疡创面肉芽组织中缺氧诱导因子-1α及血管内皮生长因子的影响]

[Effects of Chinese herbs for replenishing qi and resolving stagnation on hypoxia-inducible factor-1alpha and vascular endothelial growth factor in granulation tissue of skin ulcers in rats with diabetes].

作者信息

Que Hua-fa, Zhu Yuan-yin, Wang Yun-fei, Zhang Zhen, Xu Jie-nan, Xing Jie, Tang Han-jun

机构信息

Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.

出版信息

Zhong Xi Yi Jie He Xue Bao. 2007 Mar;5(2):165-9. doi: 10.3736/jcim20070213.

DOI:10.3736/jcim20070213

PMID:17352873

Abstract

OBJECTIVE

To explore the effects of Chinese herbs for replenishing qi and resolving stagnation on hypoxia-inducible factor-1alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF) in granulation tissue of skin ulcers in rats with syndrome of blood stasis and qi deficiency.

METHODS

Diabetic rats with back full-thickness skin lesion and syndrome of blood stasis and qi deficiency were divided in to five groups: untreated group, basic fibroblast growth factor (bFGF)-treated group, Yiqi Huayu Recipe (a recipe for replenishing qi and resolving stagnation)-treated group, Yiqi Recipe (a recipe for replenishing qi)-treated group and Huayu Recipe (a recipe for resolving stagnation)-treated group, and another eight normal rats served as normal control group. Immunohistochemical method and image analysis were used to test the expressions of HIF-1alpha and VEGF in granulation tissue of skin ulcers in rats with diabetes.

RESULTS

In the untreated group, the expression of HIF-1alpha was significantly increased and the expression of VEGF was significantly decreased as compared with those in the normal control group (P<0.01). The expression of HIF-1alpha was obviously lower (P<0.01) and the expression of VEGF was significantly higher in the four drug treated groups as compared with those in the untreated group. In the Yiqi Huayu Recipe-treated group, the expression of HIF-1alpha was obviously lower than those in the bFGF-treated, Yiqi Recipe-treated and Huayu Recipe-treated groups (P<0.05 or P<0.01), and the expression of VEGF was significantly higher than that in the bFGF-treated group (P<0.01).

CONCLUSION

Chinese herbs for replenishing qi and resolving stagnation can promote the wound healing in rats through reducing the expression of HIF-1alpha, accelerating the expression of VEGF in granulation tissue of skin ulcers in rats with diabetes and ameliorating the status of ischemia and hypoxia.

摘要

目的

探讨益气化瘀中药对气虚血瘀型大鼠皮肤溃疡创面肉芽组织中缺氧诱导因子-1α（HIF-1α）及血管内皮生长因子（VEGF）的影响。

方法

将背部全层皮肤缺损且伴有气虚血瘀证的糖尿病大鼠分为5组：未治疗组、碱性成纤维细胞生长因子（bFGF）治疗组、益气化瘀方（益气化瘀中药复方）治疗组、益气方（益气中药复方）治疗组、化瘀方（化瘀中药复方）治疗组，另取8只正常大鼠作为正常对照组。采用免疫组化法及图像分析技术检测糖尿病大鼠皮肤溃疡创面肉芽组织中HIF-1α及VEGF的表达。

结果

未治疗组与正常对照组比较，HIF-1α表达显著升高，VEGF表达显著降低（P<0.01）。4个药物治疗组与未治疗组比较，HIF-1α表达明显降低（P<0.01），VEGF表达显著升高。益气化瘀方治疗组HIF-1α表达明显低于bFGF治疗组、益气方治疗组及化瘀方治疗组（P<0.05或P<0.01），VEGF表达显著高于bFGF治疗组（P<0.01）。