Liu Qinxiu, Li Qingfeng, Zheng Shengwu, Zheng Danning
Department of Plastic and Reconstructive Surgery, the Ninth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
Arch Facial Plast Surg. 2010 Jul-Aug;12(4):263-8. doi: 10.1001/archfacial.2010.47.
To evaluate the effect of University of Wisconsin (UW) solution on composite facial flaps in dogs to offer a preservation time limit for clinical application.
The experiment included 2 parts. In part 1, 32 half facial flaps were cold stored for 12, 24, 36, and 48 hours in UW solution (experimental group) or normal saline (control group). In part 2, 8 flaps that had been cold stored in UW solution for 24 (group A, n = 3), 36 (group B, n = 3), and 48(group C, n = 2) hours were autotransplanted.
After preservation in part 1, the viability of each tissue type (skin, mucosa, muscle, blood vessel, nerve, and gland) in the experimental groups was better than that in the control group. Muscle viability decreased more quickly than did the viability of other tissue. In the experimental groups, the viability of all tissue preserved for 12 and 24 hours was better than that of tissue preserved for 36 and 48 hours. After 48 hours of preservation, tissue had good structure and integrity in the experimental group but showed degeneration in the control group. In part 2, the flap survival percentages were 100%, 100%, and 99.7% in group A; 93.2%, 95.7%, and 94.1% in group B; and 87.2% and 86.1% in group C. Six months after surgery, the dogs in group A showed contraction potential and corneal reflex.
Twenty-four hours could be considered a reference time for clinical application of UW solution flap preservation.
评估威斯康星大学（UW）溶液对犬复合面部皮瓣的影响，为临床应用提供保存时间限制。
实验包括2部分。第1部分，32个半面部皮瓣在UW溶液（实验组）或生理盐水（对照组）中冷藏12、24、36和48小时。第2部分，将8个在UW溶液中冷藏24小时（A组，n = 3）、36小时（B组，n = 3）和48小时（C组，n = 2）的皮瓣进行自体移植。
在第1部分保存后，实验组各组织类型（皮肤、黏膜、肌肉、血管、神经和腺体）的活力均优于对照组。肌肉活力比其他组织活力下降得更快。在实验组中，保存12和24小时的所有组织活力均优于保存36和48小时的组织。保存48小时后，实验组组织结构和完整性良好，而对照组出现退变。在第2部分，A组皮瓣存活百分比分别为100%、100%和99.7%；B组为93.2%、95.7%和94.1%；C组为87.2%和86.1%。术后6个月，A组犬显示出收缩潜能和角膜反射。
24小时可作为UW溶液皮瓣保存临床应用的参考时间。
