Yunnan University, Kunming, People's Republic of China.
Appl Microbiol Biotechnol. 2010 Sep;88(2):553-62. doi: 10.1007/s00253-010-2763-4. Epub 2010 Jul 20.
Flue-cured tobacco leaves (FCTL) contain abundant bacteria, and these bacteria play very important roles in the tobacco aging process. However, bacterial communities on aging FCTL are not fully understood. In this study, the total microbial genome DNA of unaged and aging flue-cured tobacco K326 were isolated using a culture-independent method, and the bacterial communities were investigated by restriction fragment length polymorphism analysis. Comparison of the number of operational taxonomic units (OTUs) between the cloned libraries from the unaged and aging FCTL showed that the microbial communities between the two groups were different. Fifty and 42 OTUs were obtained from 300 positive clones in unaged and aging FCTL, respectively. Twenty-seven species of bacteria exist in both the unaged and aging FCTL, Bacillus spp. and Pseudomonas spp. were two dominant genera in FCTL. However, 23 bacterial species were only identified from the unaged FCTL, while 15 species were only identified from the aging FCTL. Interestingly, more uncultured bacteria species were found in aging FCTL than in unaged FCTL.
烤烟烟叶（FCTL）中含有丰富的细菌，这些细菌在烟叶陈化过程中起着非常重要的作用。然而，对于陈化 FCTL 上的细菌群落还不完全了解。在这项研究中，使用非培养方法分离了未陈化和陈化烤烟 K326 的总微生物基因组 DNA，并通过限制性片段长度多态性分析研究了细菌群落。比较未陈化和陈化 FCTL 克隆文库中的操作分类单元（OTU）数量表明，两组之间的微生物群落不同。从未陈化和陈化 FCTL 的 300 个阳性克隆中分别获得了 50 和 42 个 OTU。在未陈化和陈化 FCTL 中均存在 27 种细菌，芽孢杆菌属和假单胞菌属是 FCTL 中的两个主要属。然而，只有 23 种细菌仅从未陈化的 FCTL 中鉴定出来，而只有 15 种细菌仅从陈化的 FCTL 中鉴定出来。有趣的是，陈化 FCTL 中发现的未培养细菌种类比未陈化 FCTL 中多。
