Zhou Qing, Yang Jinchu, Feng Yingjie, Yang Zongcan, Wang Yixuan, Zhang Zhan, Zhang Tingting, Liu Wenzhao, Xu YongMing, Yang Yongfeng, Huang Jihong
Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, China.
State Key Laboratory of Crop Stress Adaptation and Improvement, College of Agriculture, Henan University, Kaifeng, China.
Front Bioeng Biotechnol. 2024 Dec 6;12:1493766. doi: 10.3389/fbioe.2024.1493766. eCollection 2024.
In this study, a strain isolated from the surface of flue-cured tobacco leaves, identified as HJ-16, was applied in the solid-state fermentation of tobacco leaves. This strain, known for producing thermally stable enzymes, including amylase, cellulase, and protease, significantly improved the sensory qualities of tobacco, enhancing aromatic intensity, density, and softness, while reducing irritation. Whole-genome sequencing and functional annotation revealed that HJ-16 possesses a single circular chromosome containing genes associated with enzyme production and metabolic activities, particularly in carbohydrate metabolism and amino acid metabolism. Untargeted metabolomics analysis identified significant changes in non-volatile metabolites induced by fermentation. These metabolites were enriched in pathways related to flavonoid biosynthesis, alkaloid biosynthesis, aromatic amino acid metabolism, lipid metabolism, and carbon metabolism. Metagenomic analysis showed that became the dominant genus on the tobacco leaf surface following inoculation with HJ-16, altering the microbial community composition, reducing diversity and evenness, and enhancing microbial metabolic activity. These findings underscore the potential of as a biotechnological tool to improve tobacco leaf quality.
在本研究中，从烤烟叶片表面分离出的一株菌株（鉴定为HJ - 16）被用于烟叶的固态发酵。该菌株以产生热稳定酶（包括淀粉酶、纤维素酶和蛋白酶）而闻名，显著改善了烟草的感官品质，增强了香气强度、密度和柔软度，同时降低了刺激性。全基因组测序和功能注释表明，HJ - 16拥有一条单一的环状染色体，其中包含与酶产生和代谢活动相关的基因，特别是在碳水化合物代谢和氨基酸代谢方面。非靶向代谢组学分析确定了发酵诱导的非挥发性代谢物的显著变化。这些代谢物在与黄酮类生物合成、生物碱生物合成、芳香族氨基酸代谢、脂质代谢和碳代谢相关的途径中富集。宏基因组分析表明，接种HJ - 16后， 成为烟叶表面的优势属，改变了微生物群落组成，降低了多样性和均匀度，并增强了微生物代谢活性。这些发现强调了 作为改善烟叶品质的生物技术工具的潜力。
