Department of Biochemical Engineering, University College London, Torrington Place, London WC1E7JE, UK.
Appl Microbiol Biotechnol. 2010 Oct;88(3):671-8. doi: 10.1007/s00253-010-2726-9. Epub 2010 Jul 20.
Succinic acid is a platform molecule that has recently generated considerable interests. Production of succinate from waste orange peel and wheat straw by consolidated bioprocessing that combines cellulose hydrolysis and sugar fermentation, using a cellulolytic bacterium, Fibrobacter succinogenes S85, was studied. Orange peel contains D-limonene, which is a well-known antibacterial agent. Its effects on batch cultures of F. succinogenes S85 were examined. The minimal concentrations of limonene found to inhibit succinate and acetate generation and bacterial growth were 0.01%, 0.1%, and 0.06% (v/v), respectively. Both pre-treated orange peel by steam distillation to remove D: -limonene and intact wheat straw were used as feedstocks. Increasing the substrate concentrations of both feedstocks, from 5 to 60 g/L, elevated succinate concentration and productivity but lowered the yield. In addition, pre-treated orange peel generated greater succinate productivities than wheat straw but had similar resultant titres. The greatest succinate titres were 1.9 and 2.0 g/L for pre-treated orange peel and wheat straw, respectively. This work demonstrated that agricultural waste such as wheat straw and orange peel can be biotransformed to succinic acid by a one-step consolidated bioprocessing. Measures to increase fermentation efficiency are also discussed.
琥珀酸是一种平台分子，最近引起了相当大的关注。通过将纤维素水解和糖发酵结合起来的整合生物加工，使用纤维分解菌 Fibrobacter succinogenes S85，从废橙皮和麦秆中生产琥珀酸。橙皮中含有 D-苎烯，它是一种众所周知的抗菌剂。研究了其对纤维分解菌 S85 分批培养的影响。发现抑制琥珀酸和乙酸生成和细菌生长的苎烯最小浓度分别为 0.01%、0.1%和 0.06%（v/v）。预处理的橙皮通过蒸汽蒸馏去除 D：-苎烯和完整的麦秆都被用作原料。将两种原料的基质浓度从 5 增加到 60 g/L，提高了琥珀酸的浓度和产率，但降低了产率。此外，预处理的橙皮产生的琥珀酸比麦秆具有更高的产率，但具有相似的最终产量。预处理橙皮和麦秆的最大琥珀酸产量分别为 1.9 和 2.0 g/L。这项工作表明，农业废物如麦秆和橙皮可以通过一步整合生物加工转化为琥珀酸。还讨论了提高发酵效率的措施。
