Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Nat Prod Res. 2010 Aug;24(13):1235-42. doi: 10.1080/14786410903108951.
The chemical composition of the volatile organic compounds (VOCs) released from the leaves and flowers of Artemisia fragrans Willd. (Asteraceae) was investigated using headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography (GC) and/or GS/mass spectrometry (GC/MS). The efficiency and reliability of different fibres such as modified pencil lead and commercial polyacrylate (PA) as well as polydimethylsiloxane (PDMS) were evaluated, and pencil lead was selected to be the most suitable fibre for the extraction and sampling of the compounds. The extraction and chromatographic conditions were also optimised. Under the optimal conditions, a range of VOCs released from the leaves and flowers of A. fragrans were identified and compared. Accordingly, some bioactive components such as camphor, 1,8-cineole, alpha-terpinolene, gamma-terpinene and carvacrol were the main compounds in the VOCs released from either flowers or leaves. However, the nature and level of the volatiles in studied parts of the plant were notably varied.
采用顶空固相微萃取（HS-SPME）与气相色谱（GC）和/或气相色谱/质谱联用（GC/MS）联用的方法，研究了青蒿（菊科）叶和花中挥发性有机化合物（VOCs）的化学成分。评估了不同纤维（如改性铅笔芯和商用聚丙烯酸酯（PA）以及聚二甲基硅氧烷（PDMS））的效率和可靠性，选择铅笔芯作为提取和采样化合物的最适纤维。还优化了萃取和色谱条件。在最佳条件下，鉴定并比较了青蒿叶和花中释放的一系列 VOCs。相应地，一些生物活性成分，如樟脑、1,8-桉叶素、α-松油烯、γ-松油烯和香芹酚，是从花或叶中释放的 VOCs 的主要成分。然而，植物研究部位挥发物的性质和水平差异显著。
