Djozan Dj, Movafeghi A, Razeghi J A, Baheri T
Department of Analytical Chemistry, Faculty of Chemistry, Tabriz, Iran.
Nat Prod Res. 2008 Dec;22(18):1660-9. doi: 10.1080/14786410701877930.
A solid phase microextraction sampling method using homemade pencil-lead fibre coupled with gas chromatography was developed as a fast, easy and reliable monitoring technique to recognise the organic volatile compounds of the roots, leaves and gum of Astragalus microcephalus. The efficiency and reliability of different fibres such as homemade pencil-lead and commercial polydimethylsiloxan, as well as polyacrylate, were evaluated and the most effective fibre for recognition of these compounds was selected. Some effective experimental parameters, such as extraction time and chromatographic conditions, were examined and optimised. Under the optimised conditions, various volatile compounds such as 2-hexyn-1-ol, pentanal 3-methyl, caryophyllene, octadecane and some chlorinated components were identified in different parts of A. microcephalus. The results obtained showed that in distinct parts of A. microcephalus, the nature and level of the volatiles were varied.
开发了一种使用自制铅笔芯纤维与气相色谱联用的固相微萃取采样方法，作为一种快速、简便且可靠的监测技术，用于识别小头黄芪根、叶和胶中的有机挥发性化合物。评估了不同纤维（如自制铅笔芯、商用聚二甲基硅氧烷以及聚丙烯酸酯）的效率和可靠性，并选择了识别这些化合物最有效的纤维。研究并优化了一些有效的实验参数，如萃取时间和色谱条件。在优化条件下，在小头黄芪的不同部位鉴定出了各种挥发性化合物，如2-己炔-1-醇、3-甲基戊醛、石竹烯、十八烷和一些氯化成分。所得结果表明，在小头黄芪的不同部位，挥发物的性质和含量各不相同。
