Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, P R China.
J Biomol Struct Dyn. 2010 Oct;28(2):259-76. doi: 10.1080/07391102.2010.10507358.
The tyrosinase gene was overexpressed in HEK293 cells, and then a DNA microarray and proteomic tools were applied to detect the dysregulated genes in highly pigmented cells. The candidate genes from the microarray were compared to the yeast two-hybridization results. Computational prediction via protein-protein interaction mapping suggested the existence of 66 hub genes in melanogenesis. Most importantly, RNA binding motif protein 9 is newly detected as a putative critical melanogenesis-associated gene in this study. The approach of combining the expression data analysis and predicted protein interaction partners performed in large scales can bring more reliable gene targets for understanding pigmentation.
酪氨酸酶基因在 HEK293 细胞中过表达，然后应用 DNA 微阵列和蛋白质组学工具来检测高色素细胞中失调的基因。从微阵列中选择候选基因，并与酵母双杂交结果进行比较。通过蛋白质-蛋白质相互作用映射的计算预测提示，黑素生成中存在 66 个枢纽基因。最重要的是，在本研究中，RNA 结合基序蛋白 9 被新检测为一个潜在的关键与黑色素生成相关的基因。这种结合大规模表达数据分析和预测蛋白相互作用伙伴的方法可以为理解色素沉着提供更可靠的基因靶点。
