IDEHU (CONICET)/Department of Immunology, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina Halitus Medical Institute, Buenos Aires, Argentina.
Am J Reprod Immunol. 2011 Feb;65(2):104-9. doi: 10.1111/j.1600-0897.2010.00896.x.
Considering the potential adverse effects of anticoagulation in abortion treatment, we investigate whether antioxidants might exert the same immunoprotection. Although the fertility properties of Vitamin E have been associated with its antioxidant capacity, its effect on cytokine balance during pregnancy is still unknown.
Pregnant females from CBA/J × DBA/2 abortion model were orally supplemented with Vitamin E or inoculated intraperitoneally with enoxaparin. Foeto-placental units were scored at 14.5 days of pregnancy, and abortion rate was calculated. Cytokine placental levels were determined by enzyme-linked immunosorbent assay.
Vitamin E (15 mg/day) has been able to decrease abortion rate and to increase IL-6 placental levels, while both treatments increased vascular endothelial growth factor (VEGF) placental levels.
Vitamin E and enoxaparin are able not only to prevent foetal wastage but also to balance IL-6 and VEGF placental levels, presenting a new potential therapeutic alternative for patients with recurrent abortion not associated with thrombophilias.
考虑到抗凝在流产治疗中的潜在不良影响，我们研究抗氧化剂是否具有相同的免疫保护作用。虽然维生素 E 的生育特性与其抗氧化能力有关，但它对妊娠期间细胞因子平衡的影响尚不清楚。
CBA/J×DBA/2 流产模型的怀孕雌性动物通过口服补充维生素 E 或腹腔内注射依诺肝素进行治疗。在妊娠 14.5 天时对胎-胎盘单位进行评分，并计算流产率。通过酶联免疫吸附试验测定细胞因子胎盘水平。
维生素 E（每天 15 毫克）能够降低流产率并增加 IL-6 胎盘水平，而两种治疗方法均增加了血管内皮生长因子（VEGF）胎盘水平。
维生素 E 和依诺肝素不仅能够预防胎儿丢失，而且能够平衡 IL-6 和 VEGF 胎盘水平，为非血栓形成相关复发性流产患者提供了一种新的潜在治疗选择。
