CXCL16/CXCR6轴在人前列腺癌转移中的作用

[Role of CXCL16/CXCR6 axis in the metastasis of human prostate cancer].

作者信息

Zhou Wen-hui, Hu Wei-dong, Wu Zhou-qing, Zheng Xin-min, Wang Bi-cheng

机构信息

Reproductive Medicine Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2010 Apr 13;90(14):947-51.

PMID:20646641

Abstract

OBJECTIVE

To explore the roles of chemokine CXCL16 and its receptor CXCR6 in the directional invasion of human prostate cancer (PCa).

METHODS

The expression of CXCL16/CXCR6 in PCa samples and osseous tissues was determined by immunohistochemistry. The expression of CXCR6 in PC3 and LNCap cells was determined by reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry. Then the effects of CXCL16 upon the migration and invasion of human PC3 and LNCap cells were examined by Matrigel invasion assay.

RESULTS

The expression of CXCR6 protein was detected in all clinical PCa samples. But no CXCL16 protein was detected. Positive CXCL16 expression was observed in human osseous tissues. Both PC3 and LNCap cells expressed CXCR6 mRNA (0.38+/-0.054 vs 0.41+/-0.019 respectively) and protein. In addition, CXCL16 could promote the in vitro migration and invasion of PC3 and LNCap cell lines (invading cells 211.50+/-5.60 vs 89.25+/-3.31 respectively). Such a promoting effect of CXCL16 could not be blocked influenced by antiCXCL12 or antiCXCR4.

CONCLUSION

CXCL16/CXCR6 axis may be another independent chemokine factor playing a significant role in the metastasis of prostate cancer.

摘要

目的

探讨趋化因子CXCL16及其受体CXCR6在人前列腺癌（PCa）定向侵袭中的作用。

方法

采用免疫组织化学法检测PCa样本和骨组织中CXCL16/CXCR6的表达。采用逆转录-聚合酶链反应（RT-PCR）和免疫细胞化学法检测PC3和LNCap细胞中CXCR6的表达。然后通过基质胶侵袭试验检测CXCL16对人PC3和LNCap细胞迁移和侵袭的影响。

结果

在所有临床PCa样本中均检测到CXCR6蛋白的表达。但未检测到CXCL16蛋白。在人骨组织中观察到CXCL16阳性表达。PC3和LNCap细胞均表达CXCR6 mRNA（分别为0.38±0.054和0.41±0.019）和蛋白。此外，CXCL16可促进PC3和LNCap细胞系的体外迁移和侵袭（侵袭细胞分别为211.50±5.60和89.25±3.31）。CXCL16的这种促进作用不能被抗CXCL12或抗CXCR4阻断。