Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
FEBS Lett. 2010 Aug 20;584(16):3587-91. doi: 10.1016/j.febslet.2010.07.026. Epub 2010 Jul 18.
Treatment of Caenorhabditis elegans with apigenin, 5,7,4'-trihydroxyflavone, induces larval growth inhibition. To understand the molecular basis of apigenin-induced larval growth inhibition, the effects of apigenin on DAF-16 activity were examined. DAF-16 was activated through nuclear translocation and the mRNA level of sod-3, one of the known DAF-16 target genes, was increased upon apigenin treatment. DAF-16 activity was required for the growth inhibition, since the larval growth retardation upon apigenin treatment was suppressed in daf-16 mutants. These results indicate that apigenin acts as a stressor that activates DAF-16, which in turn inhibits larval growth.
用芹菜素（5,7,4'-三羟基黄酮）处理秀丽隐杆线虫会抑制幼虫生长。为了了解芹菜素诱导幼虫生长抑制的分子基础，研究了芹菜素对 DAF-16 活性的影响。DAF-16 通过核转位被激活，并且 sod-3 的 mRNA 水平（已知的 DAF-16 靶基因之一）在芹菜素处理后增加。DAF-16 活性是生长抑制所必需的，因为芹菜素处理引起的幼虫生长迟缓在 daf-16 突变体中被抑制。这些结果表明，芹菜素作为一种应激原激活 DAF-16，从而抑制幼虫生长。
