使用聚乙二醇处理对植物原生质体进行瞬时转化的简化程序。
Simplified procedure for transient transformation of plant protoplasts using polyethylene glycol treatment.
作者信息
Lyznik L A, Peng J Y, Hodges T K
机构信息
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907.
出版信息
Biotechniques. 1991 Mar;10(3):294-300.
PMID:2064765
Abstract
A modification of the polyethylene glycol-mediated transformation procedure which eliminates the manual polyethylene glycol dilution step is presented. A transformation mixture of protoplasts, DNA and polyethylene glycol was plated directly onto agarose blocks after incubation. The procedure was simple and fast, thereby suitable for screening the gene activity of large numbers of plasmid constructions. It has been tested for both maize and rice protoplasts.
摘要
本文介绍了一种聚乙二醇介导的转化程序的改进方法，该方法省去了手动稀释聚乙二醇的步骤。将原生质体、DNA和聚乙二醇的转化混合物孵育后直接铺在琼脂糖块上。该程序简单快速，因此适用于筛选大量质粒构建体的基因活性。它已在玉米和水稻原生质体上进行了测试。
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