[3H]-13-顺式维甲酸与人角质形成细胞中的硫氧还蛋白还原酶共价结合。

[3H]-13-cis-retinoic acid covalently binds to thioredoxin reductase in human keratinocytes.

作者信息

Schallreuter K U, Grebe T, Pittelkow M R, Wood J M

机构信息

Department of Dermatology, University of Hamburg, FRG.

出版信息

Skin Pharmacol. 1991;4(1):14-20. doi: 10.1159/000210919.

DOI:10.1159/000210919

PMID:2064786

Abstract

13-cis-Retinoic acid is a stereospecific suicide inhibitor of thioredoxin reductase. [3H]-labeled 13-cis-retinoic acid has been used to covalently label thioredoxin reductase in human keratinocytes. The acid-soluble cytosol fraction of human keratinocytes contained three radioactive proteins labeled by the addition of high-specific-activity [3H]-13-cis-retinoic acid to cell cultures. One of these proteins was identified as cytosolic keratinocyte thioredoxin reductase by fast-protein liquid chromatography and SDS-gel radioautography. The inhibition of thioredoxin reductase by 13-cis-retinoic acid may explain the known cytostatic and teratogenic properties attributed to this retinoid.

摘要

13-顺式视黄酸是硫氧还蛋白还原酶的一种立体特异性自杀抑制剂。[3H]标记的13-顺式视黄酸已被用于在人角质形成细胞中共价标记硫氧还蛋白还原酶。通过向细胞培养物中添加高比活性的[3H]-13-顺式视黄酸，人角质形成细胞的酸溶性胞质溶胶部分含有三种放射性蛋白。通过快速蛋白质液相色谱法和SDS凝胶放射自显影，其中一种蛋白被鉴定为胞质角质形成细胞硫氧还蛋白还原酶。13-顺式视黄酸对硫氧还蛋白还原酶的抑制作用可能解释了这种类维生素A已知的细胞生长抑制和致畸特性。