Takayama H, Bradley G, Lai P K, Tamura Y, Sakagami H, Tanaka A, Nonoyama M
Laboratory of Virology, Tampa Bay Research Institute, St. Petersburg, FL 33716.
AIDS Res Hum Retroviruses. 1991 Mar;7(3):349-57. doi: 10.1089/aid.1991.7.349.
We have previously shown that PC6, a natural product extracted from cones of Pinus parviflora Sieb et Zucc, can inhibit the replication of human immunodeficiency virus type 1 (HIV-1) in CD4+ T cells and in monocyte/macrophage cell lines. Here, we show by immunoprecipitation of HIV-1 proteins with a specific pooled serum that PC6 inhibited the expression of all HIV-1 proteins in CEM cells. PC6 did not affect the posttranslational processing of the HIV-1 proteins. Northern, Southern, and kinetics analyses revealed that PC6 inhibited HIV-1 reverse and forward transcription in CEM cells. Transient transfection of CEM cells with HIV-1 long terminal repeat (LTR) linked CAT DNA also showed that PC6 inhibited LTR-driven gene expression at the transcriptional level.
