Watanabe K, Momose F, Handa H, Nagata K
Department of Biomolecular Engineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Biochem Biophys Res Commun. 1995 Sep 14;214(2):318-23. doi: 10.1006/bbrc.1995.2290.
Fractions obtained from pine cone extract (PCE) of Pinus parviflora Sieb. et Zucc. have been shown to suppress the growth of influenza virus. The inhibitory effects of one of the fractions, Fraction VII, on the formation of RNA-viral protein complex and the viral RNA synthesis were investigated. The formation of M1-RNA or NP-RNA complex was inhibited when M1 or NP was preincubated with the PCE fraction. The in vitro viral RNA synthesis was inhibited by the PCE fraction, while this inhibitory effect was titrated out by the increasing concentration of M1 protein. These results suggest that the major target of the PCE fraction was M1 protein.
从赤松松果提取物（PCE）中获得的组分已被证明可抑制流感病毒的生长。对其中一个组分，即组分VII，对RNA病毒蛋白复合物形成和病毒RNA合成的抑制作用进行了研究。当M1或NP与PCE组分预孵育时，M1-RNA或NP-RNA复合物的形成受到抑制。PCE组分可抑制体外病毒RNA合成，而这种抑制作用会随着M1蛋白浓度的增加而被抵消。这些结果表明，PCE组分的主要靶点是M1蛋白。
