Shiseido Research Center, Yokohama, Japan.
J Dermatol. 2010 Aug;37(8):693-8. doi: 10.1111/j.1346-8138.2010.00867.x.
The stratum corneum (SC) is the interface of body and environment, and is continuously exposed to oxidative stress, resulting in carbonyl modification of proteins. We have developed a simple and non-invasive method to assess carbonyl protein (CP) level in the SC, applied it to various kinds of skin, and revealed a link between the stratum corneum carbonylated protein (SCCP) level and water content in the SC. The purpose of the present study is to examine the SCCP level in inflammatory skin disorders associated with xerosis. Psoriasis vulgaris (PV) and atopic dermatitis (AD) are typical inflammatory skin disorders, of which the stratum corneum shows markedly low water content. SC samples were non-invasively collected from the lesional and non-lesional areas of PV and AD by adhesive tape stripping, and their carbonyl groups were determined by reaction with fluorescein-5-thiosemicarbazide. The average fluorescence intensity of the SC was calculated as SCCP level. Higher SCCP level was observed in the lesional area of PV as compared with non-lesional area or healthy control. Lesional area of AD also exhibited higher SCCP level than corresponding non-lesional area, of which SCCP level was slightly higher than the healthy control. These data suggest the involvement of oxidative modification of the SC protein, at least in part, in generation of xerotic skin in inflammatory skin disorders as well as dry skin in healthy subjects.
表皮层（SC）是身体和环境的界面，持续暴露于氧化应激下，导致蛋白质发生羰基修饰。我们开发了一种简单、非侵入性的方法来评估 SC 中羰基蛋白（CP）的水平，将其应用于各种皮肤，并揭示了 SC 中羰基化蛋白（SCCP）水平与 SC 水分含量之间的联系。本研究的目的是研究与干燥相关的炎症性皮肤疾病中 SCCP 水平。寻常型银屑病（PV）和特应性皮炎（AD）是两种典型的炎症性皮肤疾病，其表皮层水分含量明显较低。通过胶带撕除法从 PV 和 AD 的皮损和非皮损区域无创性采集 SC 样本，并通过与荧光素-5-硫代半卡巴腙反应来测定其羰基基团。SC 的平均荧光强度被计算为 SCCP 水平。与非皮损区或健康对照组相比，PV 的皮损区 SCCP 水平更高。AD 的皮损区也表现出比相应非皮损区更高的 SCCP 水平，其 SCCP 水平略高于健康对照组。这些数据表明，氧化修饰至少部分参与了炎症性皮肤疾病中干燥皮肤的产生，以及健康受试者中干燥皮肤的产生。
