Department of Chemical Engineering, Sciences Faculty, University of Vigo (Campus Ourense), Ourense, Spain.
Lett Appl Microbiol. 2010 Aug;51(2):226-33. doi: 10.1111/j.1472-765X.2010.02882.x.
Study of the potential of Lactococcus lactis CECT-4434 as a biosurfactants and nisin (the only bacteriocin allowed to be used in the food industry) producer for industrial applications, exploiting the possibility of recovering separately both metabolites, taking into account that L. lactis is an interesting micro-organism with several applications in the food industry because it is recognized as GRAS.
The results showed the ability of this strain to produce cell-bound biosurfactants, under controlled pH, and cell-bound biosurfactants and bacteriocins, when pH was not controlled. Three extraction procedures were designed to separately recover these substances.
The strain L. lactis CECT-4434 showed to be a cell-bound biosurfactants and bacterocins producer when fermentations were carried out under uncontrolled pH. Both products can be recovered separately.
Development of a convenient tool for the extraction of cell-bound biosurfactants and bacteriocins from the fermentation broth.
研究乳球菌 CECT-4434 作为生物表面活性剂和乳链菌肽（唯一允许在食品工业中使用的细菌素）生产菌的潜力，以工业应用为目标，利用单独回收这两种代谢产物的可能性，因为乳球菌是一种在食品工业中有多种应用的有趣微生物，被认为是 GRAS。
结果表明，该菌株能够在控制 pH 值的条件下产生细胞结合型生物表面活性剂，在不控制 pH 值的条件下能够产生细胞结合型生物表面活性剂和细菌素。设计了三种提取程序来分别回收这些物质。
当发酵在非控制 pH 值下进行时，菌株 Lactococcus lactis CECT-4434 表现出生产细胞结合型生物表面活性剂和细菌素的能力。这两种产物都可以单独回收。
开发了一种从发酵液中提取细胞结合型生物表面活性剂和细菌素的简便工具。
