Department of Chemical Engineering, Sciences Faculty, University of Vigo (Campus Ourense), As Lagoas s/n, Ourense, Spain.
J Sci Food Agric. 2010 Aug 15;90(10):1726-34. doi: 10.1002/jsfa.4008.
Lactococcus lactis is an interesting microorganism with several industrial applications, particularly in the food industry. As well as being a probiotic species, L. lactis produces several metabolites with interesting properties, such as lactic acid (LA) and biosurfactants. Nevertheless, L. lactis is an especially demanding species since it has strong nutritional requirements, implying the use of complex and expensive culture media.
The results showed the potential of L. lactis CECT-4434 as a LA and biosurfactant producer. The economical cost of L. lactis cultures can be reduced by replacing the MRS medium by the use of two waste materials: trimming vine shoots as C source, and 20 g L(-1) distilled wine lees (vinasses) as N, P and micronutrient sources. From the hemicellulosic fraction, 14.3 g L(-1) LA and 1.7 mg L(-1) surfactin equivalent were achieved after 74 h (surface tension reduction of 14.4 mN m(-1)); meanwhile, a simultaneous saccharification and fermentation process allowed the generation of 10.8 g L(-1) LA and 1.5 mg L(-1) surfactin equivalent after 72 h, reducing the surface tension by 12.1 units at the end of fermentation.
Trimming vine shoots and vinasses can be used as alternative economical media for LA and cell-bound biosurfactant production.
乳球菌是一种具有多种工业应用的有趣微生物，特别是在食品工业中。乳球菌不仅是一种益生菌，还能产生多种具有有趣特性的代谢物，如乳酸（LA）和生物表面活性剂。然而，乳球菌是一种特别苛刻的物种，因为它对营养有很强的需求，这意味着需要使用复杂且昂贵的培养基。
结果表明，乳球菌 CECT-4434 具有成为 LA 和生物表面活性剂生产菌的潜力。通过使用两种废料（修剪葡萄藤嫩枝作为碳源，20 g/L 蒸馏酒酒糟作为氮、磷和微量元素源）替代 MRS 培养基，可以降低乳球菌培养的经济成本。在 74 小时后，从半纤维素部分中获得了 14.3 g/L 的 LA 和 1.7 mg/L 的表面活性剂当量（表面张力降低了 14.4 mN/m）；同时，通过同步糖化发酵过程，在 72 小时后可生成 10.8 g/L 的 LA 和 1.5 mg/L 的表面活性剂当量，在发酵结束时表面张力降低了 12.1 个单位。
修剪葡萄藤嫩枝和酒糟可以作为 LA 和细胞结合生物表面活性剂生产的替代经济型培养基。
