Wei Hong-mei, Qin Shu-kui, Yin Xiao-jin, Chen Ya-li
Postdoctoral Workstation, Nanjing General Hospital of Nanjing Command, Nanjing 210002, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Jul;30(7):1509-13.
To investigate the effect of endostar in controlling ascites tumor formation in mice.
Mouse models bearing ascites tumors were established via intraperitoneal injection of H22 and S180 cell lines. Eighty-eight ICR mice were randomly assigned into 4 groups, namely the control group (0.9% normal saline) and 3 endostar groups with 8 mg/kg endostar administration daily, every other day or every two days. The peritoneal membrane permeability of the mice was assessed using Evan blue staining. The body weight curve of mice was drawn, and the cumulative ascites volume and number of red cells and tumor cells in the malignant ascites were determined. The survival of the mice was evaluated to assess the therapeutic effect of endostar.
Compared with the control group, the mice receiving daily endostar injection showed obviously lower ascites accumulation and peritoneal capillary permeability (P<0.05) with reduced count of ascites tumor cells and red cells and tumor burden of the abdominal cavity. The mice with daily endostar injection also showed longer survival than the control group.
Continuous intraperitoneal injection can be the optimal means for endostar administration for treatment of malignant ascites.
探讨恩度对小鼠腹水瘤形成的控制作用。
通过腹腔注射H22和S180细胞系建立荷腹水瘤小鼠模型。88只ICR小鼠随机分为4组，即对照组（0.9%生理盐水）和3个恩度组，恩度给药剂量为8mg/kg，分别每日、隔日或每两日给药一次。采用伊文思蓝染色评估小鼠腹膜通透性。绘制小鼠体重曲线，测定恶性腹水中的累积腹水量、红细胞和肿瘤细胞数量。评估小鼠的生存情况以评价恩度的治疗效果。
与对照组相比，每日注射恩度的小鼠腹水积聚和腹膜毛细血管通透性明显降低（P<0.05），腹水中肿瘤细胞和红细胞计数减少，腹腔肿瘤负荷降低。每日注射恩度的小鼠生存时间也比对照组更长。
持续腹腔注射可能是恩度治疗恶性腹水的最佳给药方式。
