Gao Yue-qiu, Yao Yan, Li Man
Key Laboratory of Liver and Kidney Diseases, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 200021, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Jul;30(7):1606-9.
To investigate the expressions of perforin (PF), granzyme B (GrB), granulysin (GNLY), TNF-alpha and IFN-gamma in peripheral CD8+ T lymphocytes and their correlation to infection status in patients with chronic hepatitis B (CHB).
ALT, AST, TB and HBV DNA copy were detected to evaluate the infection status in CHB patients, with healthy volunteers serving as the control group. According to the infection status, the CHB patients were divided into 4 groups, namely normal hepatic function and high HBV DNA level group, normal hepatic function and low HBV DNA level group, abnormal hepatic function and high HBV DNA level group and abnormal hepatic function and low HBV DNA level group. The expressions of some immune effector molecules in CD8+T cells were detected by flow cytometry, and the correlations between these immune effector molecules and the infection status were analyzed.
The expressions of GrB, TNF-alpha and IFN-gamma in normal hepatic function and low HBV DNA level group were significantly higher than those in abnormal hepatic function and high HBV DNA level group (P<0.05). The expression of IFN-gamma in normal hepatic function and high HBV DNA level group was significantly higher than that in abnormal hepatic function and high HBV DNA level group (P<0.05). The expressions of PF and GNLY were similar among all the 4 groups. Positive correlations were noted between GrB, PF, GNLY, TNF-alpha and IFN-gamma.
GrB, TNF-alpha and IFN-gamma in peripheral CD8+ T cells are inversely correlated to hepatic dysfunction and HBV DNA level in CHB patients.
探讨慢性乙型肝炎（CHB）患者外周血CD8+T淋巴细胞中穿孔素（PF）、颗粒酶B（GrB）、颗粒溶素（GNLY）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）的表达及其与感染状态的相关性。
检测CHB患者的谷丙转氨酶（ALT）、谷草转氨酶（AST）、总胆红素（TB）和乙肝病毒DNA拷贝数，以评估感染状态，健康志愿者作为对照组。根据感染状态，将CHB患者分为4组，即肝功能正常且乙肝病毒DNA水平高组、肝功能正常且乙肝病毒DNA水平低组、肝功能异常且乙肝病毒DNA水平高组和肝功能异常且乙肝病毒DNA水平低组。采用流式细胞术检测CD8+T细胞中一些免疫效应分子的表达，并分析这些免疫效应分子与感染状态之间的相关性。
肝功能正常且乙肝病毒DNA水平低组中GrB、TNF-α和IFN-γ的表达显著高于肝功能异常且乙肝病毒DNA水平高组（P<0.05）。肝功能正常且乙肝病毒DNA水平高组中IFN-γ的表达显著高于肝功能异常且乙肝病毒DNA水平高组（P<0.05）。4组中PF和GNLY的表达相似。GrB、PF、GNLY、TNF-α和IFN-γ之间呈正相关。
CHB患者外周血CD8+T细胞中的GrB、TNF-α和IFN-γ与肝功能障碍及乙肝病毒DNA水平呈负相关。
