Centro Cardiologico Monzino, IRCCS, Dept of Cardiovascular Sciences, University of Milan, Milan, Italy.
Eur Respir J. 2011 Apr;37(4):841-7. doi: 10.1183/09031936.00045910. Epub 2010 Jul 22.
Surfactant derived protein B (SPB) and plasma receptor for advanced glycation end products (RAGE) have been proposed as markers of lung injury. The former is produced specifically by pneumocytes while RAGE production is present in several body tissues. Cardiopulmonary bypass (CPB) generates a transient lung injury. We measured SPB and RAGE in plasma before surgery and after CPB, as well as 24 h and 48 h later. We analysed plasma samples from 20 subjects scheduled for elective coronary artery bypass grafting. We performed a quantitative analysis of plasma levels of RAGE and SPB mature form (8 kDa) by ELISA and a semi-quantitative analysis of SPB immature form (~ 40 kDa) by Western blotting. Surgery procedures were uneventful. After CPB RAGE median (75th-25th interquartile difference) increased from 633 (539) pg·mL⁻¹ to 1,362 (557) pg·mL⁻¹ (p < 0.01), while mature SPB increased from 5,587 (3,089) ng·mL⁻¹ to 20,307 (19,873) ng·mL⁻¹ (p < 0.01). RAGE and mature SPB returned to normal values within 48 h. This behaviour was confirmed when RAGE and SPB were normalised for protein content. Parallel changes were observed for immature SPB. Plasma RAGE and SPBs are sensitive and rapid markers of lung distress.
表面活性物质相关蛋白 B（SPB）和血浆晚期糖基化终产物受体（RAGE）被提议作为肺损伤的标志物。前者是由肺细胞特异性产生的，而 RAGE 的产生存在于几种身体组织中。体外循环（CPB）会导致短暂的肺损伤。我们在手术前、CPB 后以及之后 24 小时和 48 小时测量了血浆中的 SPB 和 RAGE。我们分析了 20 名计划进行选择性冠状动脉旁路移植术的患者的血浆样本。我们通过 ELISA 对 RAGE 和 SPB 成熟形式（8 kDa）进行了定量分析，通过 Western blot 对半定量分析了 SPB 不成熟形式（~40 kDa）。手术过程顺利。CPB 后，RAGE 中位数（75%至 25%四分位差）从 633（539）pg·mL⁻¹增加到 1362（557）pg·mL⁻¹（p<0.01），而成熟 SPB 从 5587（3089）ng·mL⁻¹增加到 20307（19873）ng·mL⁻¹（p<0.01）。RAGE 和成熟 SPB 在 48 小时内恢复正常。当 RAGE 和 SPB 按蛋白含量归一化时，也证实了这种行为。不成熟 SPB 也观察到了类似的变化。血浆 RAGE 和 SPB 是肺损伤的敏感和快速标志物。
