Department of Cytopathology, University General Hospital Attikon, School of Medicine, National and Kapodistrian University of Athens, Rimini 1, 12462, Chaidari, Greece.
Anticancer Res. 2010 Jun;30(6):1969-75.
Real-time PCR-based molecular diagnostic techniques were used for the comparative identification of the most common KRAS and BRAF mutations from methanol liquid-based cytology samples and matched, formalin-fixed and paraffin-embedded tissue samples. Methanol-based fixation and preservation of brushing samples of colorectal carcinomas could contribute to sample collection, short-term storage and subsequent molecular analysis, by improving workflow and enhancing the identification of putative mutations.
基于实时 PCR 的分子诊断技术被用于比较鉴定甲醇液基细胞学样本和匹配的福尔马林固定石蜡包埋组织样本中最常见的 KRAS 和 BRAF 突变。基于甲醇的固定和保存结直肠癌细胞刷检样本可以通过改进工作流程和增强对可能突变的识别来促进样本采集、短期储存和随后的分子分析。
