Division of Hearing and Balance Research, National Institute of Sensory Organs, National Tokyo Medical Center, Meguro, Tokyo 152-8902, Japan.
Anticancer Res. 2010 Jun;30(6):2005-10.
This study investigated the existence of stem-like cells in established head and neck squamous cell carcinoma (HNSCC) lines, HSC3 and HSC4. Flow cytometric analysis confirmed the presence of side population (SP) cells excluding Hoechst 33342 in HSC4 cells (0.37+/-0.06%) but not HSC3 cells in a reserpine-sensitive manner. After sorting, the SP cells generated both SP and main population (MP) cells in culture while MP cells generated MP cells only. Higher expression of stem cell markers was detected in SP than in MP cells. These results suggest that cancer stem-like cells exist in head and neck squamous cell carcinoma.
本研究调查了已建立的头颈部鳞状细胞癌（HNSCC）细胞系 HSC3 和 HSC4 中是否存在干细胞样细胞。流式细胞术分析证实，HSC4 细胞中存在侧群（SP）细胞，这些细胞能够排除 Hoechst 33342，呈利血平敏感方式（0.37+/-0.06%），但 HSC3 细胞中不存在。经过分选后，SP 细胞在培养中既能生成 SP 细胞又能生成主群（MP）细胞，而 MP 细胞只能生成 MP 细胞。在 SP 细胞中检测到更高水平的干细胞标记物的表达。这些结果表明，头颈部鳞状细胞癌中存在癌症干细胞样细胞。
