Jalbuena R C, Tyrer H W
Dept. of Elect. and Computer Eng., Univ. of Missouri, Columbia.
Biomed Sci Instrum. 1991;27:9-19.
The Cell Deposition System (CDS) analyzes and deposits biological cells to specific locations on microscope slides. This research will determine the accuracy of the CDS by comparing the stored computer data with the cells placement on the microscope slide. Two independent maps (Control 1 and Control 2) of cell placement for each slide were made, each map to provide a control for the other. The fluorescence data (cell sequence data) was compared to each of the control maps. For the slide, CT4, the error between maps was 13.6% mismatches, between Control 2 and the fluorescence data 32.1% mismatches. Comparing the maps 3/16 rows were perfectly matched; Whereas, comparing fluorescence data to maps 1/16 express perfect matches. Depositing cells in this manner is remarkable accurate. Nevertheless work remains to prevent the "disappearance" of cells, their adjacent positional invasion, and their clustering. This technique provides a superior preparative method for sample archival storage and for further automated multiparametric microscope assessment.
细胞沉积系统（CDS）可分析生物细胞并将其沉积到显微镜载玻片上的特定位置。本研究将通过比较存储的计算机数据与显微镜载玻片上细胞的放置情况来确定CDS的准确性。为每张载玻片制作了两个独立的细胞放置图谱（对照1和对照2），每个图谱作为另一个的对照。将荧光数据（细胞序列数据）与每个对照图谱进行比较。对于CT4载玻片，图谱之间的误差为13.6%的错配，对照2与荧光数据之间为32.1%的错配。比较图谱时，3/16行完全匹配；而将荧光数据与图谱比较时，1/16表现出完全匹配。以这种方式沉积细胞非常准确。然而，仍需努力防止细胞“消失”、其相邻位置的侵入以及细胞聚集。这项技术为样本档案存储和进一步的自动化多参数显微镜评估提供了一种优越的制备方法。
